Proteomic profiling of serum of women with BI-RADS 1 to 5: identification of potential complementary biomarkers for early detection of breast cancer
Mammography remains the gold standard for the screening of breast cancer (BrCa) despite its shortcomings. Cancer antigen 15-3, an FDA-approved biomarker, is most useful as a treatment response and recurrence monitoring tool rather than for early detection of BrCa. Given this, we aimed to screen for...
Saved in:
| Main Authors: | , , , , |
|---|---|
| Format: | Article |
| Language: | en |
| Published: |
Penerbit Universiti Kebangsaan Malaysia
2024
|
| Online Access: | http://journalarticle.ukm.my/24332/1/SL%203.pdf http://journalarticle.ukm.my/24332/ https://www.ukm.my/jsm/english_journals/vol53num8_2024/contentsVol53num8_2024.html |
| Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
| _version_ | 1834434923475238912 |
|---|---|
| author | Jacqueline, Jaime Jayapalan Jane, Christina Vellan Tania Islam, Nur Aishah Mohd Taib, Karuthan Chinna, |
| author_facet | Jacqueline, Jaime Jayapalan Jane, Christina Vellan Tania Islam, Nur Aishah Mohd Taib, Karuthan Chinna, |
| author_sort | Jacqueline, Jaime Jayapalan |
| building | Tun Sri Lanang Library |
| collection | Institutional Repository |
| content_provider | Universiti Kebangsaan Malaysia |
| content_source | UKM Journal Article Repository |
| continent | Asia |
| country | Malaysia |
| description | Mammography remains the gold standard for the screening of breast cancer (BrCa) despite its shortcomings. Cancer antigen 15-3, an FDA-approved biomarker, is most useful as a treatment response and recurrence monitoring tool rather than for early detection of BrCa. Given this, we aimed to screen for potential complementary diagnostic protein biomarkers in the serum of women with BI-RADS 1 to 5. Individual neat sera of women with BI-RADS 1 to 5 (N = 33) were subjected to two-dimensional electrophoresis (2-DE) for the separation of proteins. Comparative analysis of the 2-DE silver-stained gel images was performed using Progenesis SameSpots software. The identification of protein spots of interest was determined following tandem MS/MS analysis and database search using either MASCOT or X! Tandem Vengeance search engines. Data are available via ProteomeXchange with the identifier PXD040427. The Bioinformatics tools of The Database for Annotation, Visualization, and Integrated Discovery were used for the functional annotation of the proteins of altered abundance. A total of 8 non-redundant proteins including albumin, apolipoprotein A-I, apolipoprotein A-II, clusterin, complement C3, immunoglobulin kappa constant, kininogen-1, and leucine-rich alpha-2 glycoprotein were found significantly overexpressed in the sera of women with BI-RADS 4 and/or 5 (p < 0.01, FC ≥ 2). Functional annotation of the significantly differentially expressed proteins showed their possible roles in the development of BrCa. The identified protein signatures are potential biomarkers for use in complement with mammography for improved detection of BrCa at an early stage. |
| format | Article |
| id | my-ukm.journal.24332 |
| institution | Universiti Kebangsaan Malaysia |
| language | en |
| publishDate | 2024 |
| publisher | Penerbit Universiti Kebangsaan Malaysia |
| record_format | eprints |
| spelling | my-ukm.journal.243322024-10-11T02:34:59Z http://journalarticle.ukm.my/24332/ Proteomic profiling of serum of women with BI-RADS 1 to 5: identification of potential complementary biomarkers for early detection of breast cancer Jacqueline, Jaime Jayapalan Jane, Christina Vellan Tania Islam, Nur Aishah Mohd Taib, Karuthan Chinna, Mammography remains the gold standard for the screening of breast cancer (BrCa) despite its shortcomings. Cancer antigen 15-3, an FDA-approved biomarker, is most useful as a treatment response and recurrence monitoring tool rather than for early detection of BrCa. Given this, we aimed to screen for potential complementary diagnostic protein biomarkers in the serum of women with BI-RADS 1 to 5. Individual neat sera of women with BI-RADS 1 to 5 (N = 33) were subjected to two-dimensional electrophoresis (2-DE) for the separation of proteins. Comparative analysis of the 2-DE silver-stained gel images was performed using Progenesis SameSpots software. The identification of protein spots of interest was determined following tandem MS/MS analysis and database search using either MASCOT or X! Tandem Vengeance search engines. Data are available via ProteomeXchange with the identifier PXD040427. The Bioinformatics tools of The Database for Annotation, Visualization, and Integrated Discovery were used for the functional annotation of the proteins of altered abundance. A total of 8 non-redundant proteins including albumin, apolipoprotein A-I, apolipoprotein A-II, clusterin, complement C3, immunoglobulin kappa constant, kininogen-1, and leucine-rich alpha-2 glycoprotein were found significantly overexpressed in the sera of women with BI-RADS 4 and/or 5 (p < 0.01, FC ≥ 2). Functional annotation of the significantly differentially expressed proteins showed their possible roles in the development of BrCa. The identified protein signatures are potential biomarkers for use in complement with mammography for improved detection of BrCa at an early stage. Penerbit Universiti Kebangsaan Malaysia 2024 Article PeerReviewed application/pdf en http://journalarticle.ukm.my/24332/1/SL%203.pdf Jacqueline, Jaime Jayapalan and Jane, Christina Vellan and Tania Islam, and Nur Aishah Mohd Taib, and Karuthan Chinna, (2024) Proteomic profiling of serum of women with BI-RADS 1 to 5: identification of potential complementary biomarkers for early detection of breast cancer. Sains Malaysiana, 53 (8). pp. 1749-1765. ISSN 0126-6039 https://www.ukm.my/jsm/english_journals/vol53num8_2024/contentsVol53num8_2024.html |
| spellingShingle | Jacqueline, Jaime Jayapalan Jane, Christina Vellan Tania Islam, Nur Aishah Mohd Taib, Karuthan Chinna, Proteomic profiling of serum of women with BI-RADS 1 to 5: identification of potential complementary biomarkers for early detection of breast cancer |
| title | Proteomic profiling of serum of women with BI-RADS 1 to 5: identification of potential complementary biomarkers for early detection of breast cancer |
| title_full | Proteomic profiling of serum of women with BI-RADS 1 to 5: identification of potential complementary biomarkers for early detection of breast cancer |
| title_fullStr | Proteomic profiling of serum of women with BI-RADS 1 to 5: identification of potential complementary biomarkers for early detection of breast cancer |
| title_full_unstemmed | Proteomic profiling of serum of women with BI-RADS 1 to 5: identification of potential complementary biomarkers for early detection of breast cancer |
| title_short | Proteomic profiling of serum of women with BI-RADS 1 to 5: identification of potential complementary biomarkers for early detection of breast cancer |
| title_sort | proteomic profiling of serum of women with bi-rads 1 to 5: identification of potential complementary biomarkers for early detection of breast cancer |
| url | http://journalarticle.ukm.my/24332/1/SL%203.pdf http://journalarticle.ukm.my/24332/ https://www.ukm.my/jsm/english_journals/vol53num8_2024/contentsVol53num8_2024.html |
| url_provider | http://journalarticle.ukm.my/ |