GC-MS analysis and antibacterial activity of ethanolic and water extracts of Malaysian Heterotrigona itama propolis against selected human pathogenic bacteria
Despite the growing interest in the therapeutic potential of propolis, limited attention has been paid to the chemical composition and biological activity of water extract propolis produced by Malaysian stingless bees. Thus, this study aimed to determine the phytochemical composition and antibacteri...
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Penerbit Universiti Kebangsaan Malaysia
2023
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| Online Access: | http://journalarticle.ukm.my/22407/1/MAD%209.pdf http://journalarticle.ukm.my/22407/ https://jms.mabjournal.com/index.php/mab/index |
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| author | Rozaini Mohd Zohdi, Muhammad Amirul Adli, Hannis Fadzillah Mohsin, Shahida Muhamad Mokhtar, Anis Low Muhammad Low, Awang Hazmi Awang Junaidi, Dzu Hendra Ja Jahrudin, |
| author_facet | Rozaini Mohd Zohdi, Muhammad Amirul Adli, Hannis Fadzillah Mohsin, Shahida Muhamad Mokhtar, Anis Low Muhammad Low, Awang Hazmi Awang Junaidi, Dzu Hendra Ja Jahrudin, |
| author_sort | Rozaini Mohd Zohdi, |
| building | Tun Sri Lanang Library |
| collection | Institutional Repository |
| content_provider | Universiti Kebangsaan Malaysia |
| content_source | UKM Journal Article Repository |
| continent | Asia |
| country | Malaysia |
| description | Despite the growing interest in the therapeutic potential of propolis, limited attention has been paid to the chemical composition and biological activity of water extract propolis produced by Malaysian stingless bees. Thus, this study aimed to determine the phytochemical composition and antibacterial potential of ethanolic extract propolis (EEP) and water extract propolis (WEP) of the stingless bee species, Heterotrigona itama against ten pathogenic bacteria. The phytochemical analysis of the extracts was carried out using the gas chromatography-mass spectrometry (GC-MS) technique. The antibacterial activity was determined using the disc-diffusion, minimum inhibition concentration (MIC), and minimum bactericidal concentration (MBC) methods. The GC-MS analysis of EEP exhibited four volatile compounds including hexamethylcyclotrisiloxane, 2-hydroxy-2-cyclopenten-1-one, 2-coumaranone, and diethyl bis(trimethylsilyl) ester silicic acid. However, only two compounds were identified in WEP, consisting of 2-(acetoxymethyl)-3-(methoxycarbonyl)biphenylene, and hexamethylcyclotrisiloxane. EEP showed the highest antibacterial activity against all Gram-positive bacteria (Bacillus subtilis, Enterococcus faecalis, Enterococcus faecium, Micrococcus luteus, Staphylococcus aureus, Streptococcus mutans) with values of the inhibition zones ranging from 7 to 10 mm. However, both extracts showed no antibacterial activity against Gram-negative bacteria, except WEP, which displayed an inhibition zone of 9.33 ± 1.53 mm against Escherichia coli. Meanwhile, EEP showed the lowest MIC and MBC values against M. luteus at 70 and 280 μg/mL, respectively. The results revealed the presence of several volatile compounds in the EEP of H. itama which could contribute to its antibacterial activity, particularly against Gram-positive bacteria. |
| format | Article |
| id | my-ukm.journal.22407 |
| institution | Universiti Kebangsaan Malaysia |
| language | en |
| publishDate | 2023 |
| publisher | Penerbit Universiti Kebangsaan Malaysia |
| record_format | eprints |
| spelling | my-ukm.journal.224072023-10-24T07:35:13Z http://journalarticle.ukm.my/22407/ GC-MS analysis and antibacterial activity of ethanolic and water extracts of Malaysian Heterotrigona itama propolis against selected human pathogenic bacteria Rozaini Mohd Zohdi, Muhammad Amirul Adli, Hannis Fadzillah Mohsin, Shahida Muhamad Mokhtar, Anis Low Muhammad Low, Awang Hazmi Awang Junaidi, Dzu Hendra Ja Jahrudin, Despite the growing interest in the therapeutic potential of propolis, limited attention has been paid to the chemical composition and biological activity of water extract propolis produced by Malaysian stingless bees. Thus, this study aimed to determine the phytochemical composition and antibacterial potential of ethanolic extract propolis (EEP) and water extract propolis (WEP) of the stingless bee species, Heterotrigona itama against ten pathogenic bacteria. The phytochemical analysis of the extracts was carried out using the gas chromatography-mass spectrometry (GC-MS) technique. The antibacterial activity was determined using the disc-diffusion, minimum inhibition concentration (MIC), and minimum bactericidal concentration (MBC) methods. The GC-MS analysis of EEP exhibited four volatile compounds including hexamethylcyclotrisiloxane, 2-hydroxy-2-cyclopenten-1-one, 2-coumaranone, and diethyl bis(trimethylsilyl) ester silicic acid. However, only two compounds were identified in WEP, consisting of 2-(acetoxymethyl)-3-(methoxycarbonyl)biphenylene, and hexamethylcyclotrisiloxane. EEP showed the highest antibacterial activity against all Gram-positive bacteria (Bacillus subtilis, Enterococcus faecalis, Enterococcus faecium, Micrococcus luteus, Staphylococcus aureus, Streptococcus mutans) with values of the inhibition zones ranging from 7 to 10 mm. However, both extracts showed no antibacterial activity against Gram-negative bacteria, except WEP, which displayed an inhibition zone of 9.33 ± 1.53 mm against Escherichia coli. Meanwhile, EEP showed the lowest MIC and MBC values against M. luteus at 70 and 280 μg/mL, respectively. The results revealed the presence of several volatile compounds in the EEP of H. itama which could contribute to its antibacterial activity, particularly against Gram-positive bacteria. Penerbit Universiti Kebangsaan Malaysia 2023 Article PeerReviewed application/pdf en http://journalarticle.ukm.my/22407/1/MAD%209.pdf Rozaini Mohd Zohdi, and Muhammad Amirul Adli, and Hannis Fadzillah Mohsin, and Shahida Muhamad Mokhtar, and Anis Low Muhammad Low, and Awang Hazmi Awang Junaidi, and Dzu Hendra Ja Jahrudin, (2023) GC-MS analysis and antibacterial activity of ethanolic and water extracts of Malaysian Heterotrigona itama propolis against selected human pathogenic bacteria. Malaysian Applied Biology, 52 (2). pp. 77-84. ISSN 0126-8643 https://jms.mabjournal.com/index.php/mab/index |
| spellingShingle | Rozaini Mohd Zohdi, Muhammad Amirul Adli, Hannis Fadzillah Mohsin, Shahida Muhamad Mokhtar, Anis Low Muhammad Low, Awang Hazmi Awang Junaidi, Dzu Hendra Ja Jahrudin, GC-MS analysis and antibacterial activity of ethanolic and water extracts of Malaysian Heterotrigona itama propolis against selected human pathogenic bacteria |
| title | GC-MS analysis and antibacterial activity of ethanolic and water extracts of Malaysian Heterotrigona itama propolis against selected human pathogenic bacteria |
| title_full | GC-MS analysis and antibacterial activity of ethanolic and water extracts of Malaysian Heterotrigona itama propolis against selected human pathogenic bacteria |
| title_fullStr | GC-MS analysis and antibacterial activity of ethanolic and water extracts of Malaysian Heterotrigona itama propolis against selected human pathogenic bacteria |
| title_full_unstemmed | GC-MS analysis and antibacterial activity of ethanolic and water extracts of Malaysian Heterotrigona itama propolis against selected human pathogenic bacteria |
| title_short | GC-MS analysis and antibacterial activity of ethanolic and water extracts of Malaysian Heterotrigona itama propolis against selected human pathogenic bacteria |
| title_sort | gc-ms analysis and antibacterial activity of ethanolic and water extracts of malaysian heterotrigona itama propolis against selected human pathogenic bacteria |
| url | http://journalarticle.ukm.my/22407/1/MAD%209.pdf http://journalarticle.ukm.my/22407/ https://jms.mabjournal.com/index.php/mab/index |
| url_provider | http://journalarticle.ukm.my/ |