Monosaccharide profile and antioxidant activities of sulphated polysaccharide extracted from Indonesian brown seaweed (Sargassum sp.)
This research was conducted to determine the monosaccharide profile and antioxidant activities of the sulphated polysaccharide fucoidan, extracted from Sargassum sp. harvested in Batam, Indonesia. Crude fucoidan (Fsar) was extracted using low pH (pH 2) acid method, obtaining a yield of 5.5% (w/w...
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Penerbit Universiti Kebangsaan Malaysia
2022
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| Online Access: | http://journalarticle.ukm.my/20865/1/7.pdf http://journalarticle.ukm.my/20865/ https://www.ukm.my/jsm/malay_journals/jilid51bil10_2022/KandunganJilid51Bil10_2022.html |
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| author | Lim, Seng Joe Sharifah Habibah Syed Khalafu, Böhmdorfer, Stefan Rosenau, Thomas Mohamad Yusof Maskat, Noor Soffalina Sofian Seng, Noorul Syuhada Mohd Razali, Wan Aida Wan Mustapha, |
| author_facet | Lim, Seng Joe Sharifah Habibah Syed Khalafu, Böhmdorfer, Stefan Rosenau, Thomas Mohamad Yusof Maskat, Noor Soffalina Sofian Seng, Noorul Syuhada Mohd Razali, Wan Aida Wan Mustapha, |
| author_sort | Lim, Seng Joe |
| building | Tun Sri Lanang Library |
| collection | Institutional Repository |
| content_provider | Universiti Kebangsaan Malaysia |
| content_source | UKM Journal Article Repository |
| continent | Asia |
| country | Malaysia |
| description | This research was conducted to determine the monosaccharide profile and antioxidant activities of the sulphated
polysaccharide fucoidan, extracted from Sargassum sp. harvested in Batam, Indonesia. Crude fucoidan (Fsar) was
extracted using low pH (pH 2) acid method, obtaining a yield of 5.5% (w/w), and the chemical properties of Fsar were
subsequently compared to commercial fucoidan (Fcomm), obtained from Japan. Sulphate contents were determined
using spectrophotometric analysis, where Fsar (5.21±0.35%) and Fcomm (8.80±0.19%) differed significantly (p<0.05).
Monosaccharide profiling was performed using methanolysis and gas chromatography-flame ionisation detector
(GC-FID). Fsar contained fucose (138.34 µg/g), 4-O-methyl-glucuronic acid (105.39 µg/g), galactose (70.49 µg/g)
and glucuronic acid (30.60 µg/g) while Fcomm contained fucose (520 µg/g) and glucuronic acid (62.93 µg/g) as its
main composition. Antioxidant activities were analysed through 2,2-diphenyl-1-picrylhydrazyl (DPPH), superoxide
anion (SOA) and hydroxyl radical (·OH) scavenging activities. DPPH scavenging activity of Fsar (40.67±4.0%) was
significantly higher (p<0.05) than Fcomm (10.11±1.0%), while SOA and ·OH scavenging activities showed no significant
difference (p>0.05) between Fsar and Fcomm. Fsar showed significant differences (p<0.05) in chemical properties and DPPH
scavenging activities compared to that of Fcomm. Fucoidan from Southeast Asia waters has the potential for application
as functional ingredients. |
| format | Article |
| id | my-ukm.journal.20865 |
| institution | Universiti Kebangsaan Malaysia |
| language | en |
| publishDate | 2022 |
| publisher | Penerbit Universiti Kebangsaan Malaysia |
| record_format | eprints |
| spelling | my-ukm.journal.208652022-12-22T08:45:02Z http://journalarticle.ukm.my/20865/ Monosaccharide profile and antioxidant activities of sulphated polysaccharide extracted from Indonesian brown seaweed (Sargassum sp.) Lim, Seng Joe Sharifah Habibah Syed Khalafu, Böhmdorfer, Stefan Rosenau, Thomas Mohamad Yusof Maskat, Noor Soffalina Sofian Seng, Noorul Syuhada Mohd Razali, Wan Aida Wan Mustapha, This research was conducted to determine the monosaccharide profile and antioxidant activities of the sulphated polysaccharide fucoidan, extracted from Sargassum sp. harvested in Batam, Indonesia. Crude fucoidan (Fsar) was extracted using low pH (pH 2) acid method, obtaining a yield of 5.5% (w/w), and the chemical properties of Fsar were subsequently compared to commercial fucoidan (Fcomm), obtained from Japan. Sulphate contents were determined using spectrophotometric analysis, where Fsar (5.21±0.35%) and Fcomm (8.80±0.19%) differed significantly (p<0.05). Monosaccharide profiling was performed using methanolysis and gas chromatography-flame ionisation detector (GC-FID). Fsar contained fucose (138.34 µg/g), 4-O-methyl-glucuronic acid (105.39 µg/g), galactose (70.49 µg/g) and glucuronic acid (30.60 µg/g) while Fcomm contained fucose (520 µg/g) and glucuronic acid (62.93 µg/g) as its main composition. Antioxidant activities were analysed through 2,2-diphenyl-1-picrylhydrazyl (DPPH), superoxide anion (SOA) and hydroxyl radical (·OH) scavenging activities. DPPH scavenging activity of Fsar (40.67±4.0%) was significantly higher (p<0.05) than Fcomm (10.11±1.0%), while SOA and ·OH scavenging activities showed no significant difference (p>0.05) between Fsar and Fcomm. Fsar showed significant differences (p<0.05) in chemical properties and DPPH scavenging activities compared to that of Fcomm. Fucoidan from Southeast Asia waters has the potential for application as functional ingredients. Penerbit Universiti Kebangsaan Malaysia 2022-10 Article PeerReviewed application/pdf en http://journalarticle.ukm.my/20865/1/7.pdf Lim, Seng Joe and Sharifah Habibah Syed Khalafu, and Böhmdorfer, Stefan and Rosenau, Thomas and Mohamad Yusof Maskat, and Noor Soffalina Sofian Seng, and Noorul Syuhada Mohd Razali, and Wan Aida Wan Mustapha, (2022) Monosaccharide profile and antioxidant activities of sulphated polysaccharide extracted from Indonesian brown seaweed (Sargassum sp.). Sains Malaysiana, 51 (10). pp. 3195-3202. ISSN 0126-6039 https://www.ukm.my/jsm/malay_journals/jilid51bil10_2022/KandunganJilid51Bil10_2022.html |
| spellingShingle | Lim, Seng Joe Sharifah Habibah Syed Khalafu, Böhmdorfer, Stefan Rosenau, Thomas Mohamad Yusof Maskat, Noor Soffalina Sofian Seng, Noorul Syuhada Mohd Razali, Wan Aida Wan Mustapha, Monosaccharide profile and antioxidant activities of sulphated polysaccharide extracted from Indonesian brown seaweed (Sargassum sp.) |
| title | Monosaccharide profile and antioxidant activities of sulphated polysaccharide extracted from Indonesian brown seaweed (Sargassum sp.) |
| title_full | Monosaccharide profile and antioxidant activities of sulphated polysaccharide extracted from Indonesian brown seaweed (Sargassum sp.) |
| title_fullStr | Monosaccharide profile and antioxidant activities of sulphated polysaccharide extracted from Indonesian brown seaweed (Sargassum sp.) |
| title_full_unstemmed | Monosaccharide profile and antioxidant activities of sulphated polysaccharide extracted from Indonesian brown seaweed (Sargassum sp.) |
| title_short | Monosaccharide profile and antioxidant activities of sulphated polysaccharide extracted from Indonesian brown seaweed (Sargassum sp.) |
| title_sort | monosaccharide profile and antioxidant activities of sulphated polysaccharide extracted from indonesian brown seaweed (sargassum sp.) |
| url | http://journalarticle.ukm.my/20865/1/7.pdf http://journalarticle.ukm.my/20865/ https://www.ukm.my/jsm/malay_journals/jilid51bil10_2022/KandunganJilid51Bil10_2022.html |
| url_provider | http://journalarticle.ukm.my/ |