Fatty acids and terpenoids from Canarium odontophyllum MIQ. Leaf and their antioxidant and cytotoxic effects on UVB-induced immortalized human keratinocytes cells (HACAT)
The study evaluated the antioxidant capacity of hexane extract of Canarium odontophyllum Miq. leaf; its fatty acids and terpenoids content; and cytotoxic effects on UVB-induced human keratinocytes (HaCaT). FRAP assay was used to determine antioxidant capacity. GC-MS analysis to identify the fatt...
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| Language: | en |
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Penerbit Universiti Kebangsaan Malaysia
2022
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| Online Access: | http://journalarticle.ukm.my/20742/1/079-087%2BMAB%2B2377.pdf http://journalarticle.ukm.my/20742/ https://jms.mabjournal.com/index.php/mab/issue/view/46 |
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| author | Muhammad Wahizul Haswan Abdul Aziz, Dayang Fredalina Basri, Siti Fathiah Masre, Ahmad Rohi Ghazali, |
| author_facet | Muhammad Wahizul Haswan Abdul Aziz, Dayang Fredalina Basri, Siti Fathiah Masre, Ahmad Rohi Ghazali, |
| author_sort | Muhammad Wahizul Haswan Abdul Aziz, |
| building | Tun Sri Lanang Library |
| collection | Institutional Repository |
| content_provider | Universiti Kebangsaan Malaysia |
| content_source | UKM Journal Article Repository |
| continent | Asia |
| country | Malaysia |
| description | The study evaluated the antioxidant capacity of hexane extract of Canarium odontophyllum Miq. leaf; its fatty acids and
terpenoids content; and cytotoxic effects on UVB-induced human keratinocytes (HaCaT). FRAP assay was used to determine
antioxidant capacity. GC-MS analysis to identify the fatty acids and terpenoids’ in the hexane extract of Canarium odontophyllum
Miq. leaf. MTT (3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide) assay was carried out to measure the cytotoxic
effects of the extract on UVB-induced human keratinocytes. Serial doses of up to 1000 µg/mL extract were administered
before UVB irradiation of the cells. FRAP assay showed the extract was found to exhibit antioxidant activity but no significant
difference in ascorbic acid equivalent antioxidant capacity (AEAC) between dose 500 µg/mL (5.00 ± 0.35 AEAC) and 1000 µg/
mL (5.70 ± 0.29 AEAC) extract. GC-MS analysis showed the extract contained 88.93% of fatty acids and terpenoids, especially
n-hexadecanoic acid, spathulenol, and phytol. MTT assay showed no IC50 value for the tested extract dose on UVB-induced
HaCaT. Thus, the results suggest the potential application of hexane extract of C. odontophyllum Miq. leaf in terpenoids’ studies.
In-depth research and isolation of compounds of interest should be done to develop it as a viable medical phytotherapeutic agent. |
| format | Article |
| id | my-ukm.journal.20742 |
| institution | Universiti Kebangsaan Malaysia |
| language | en |
| publishDate | 2022 |
| publisher | Penerbit Universiti Kebangsaan Malaysia |
| record_format | eprints |
| spelling | my-ukm.journal.207422022-12-14T07:25:43Z http://journalarticle.ukm.my/20742/ Fatty acids and terpenoids from Canarium odontophyllum MIQ. Leaf and their antioxidant and cytotoxic effects on UVB-induced immortalized human keratinocytes cells (HACAT) Muhammad Wahizul Haswan Abdul Aziz, Dayang Fredalina Basri, Siti Fathiah Masre, Ahmad Rohi Ghazali, The study evaluated the antioxidant capacity of hexane extract of Canarium odontophyllum Miq. leaf; its fatty acids and terpenoids content; and cytotoxic effects on UVB-induced human keratinocytes (HaCaT). FRAP assay was used to determine antioxidant capacity. GC-MS analysis to identify the fatty acids and terpenoids’ in the hexane extract of Canarium odontophyllum Miq. leaf. MTT (3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide) assay was carried out to measure the cytotoxic effects of the extract on UVB-induced human keratinocytes. Serial doses of up to 1000 µg/mL extract were administered before UVB irradiation of the cells. FRAP assay showed the extract was found to exhibit antioxidant activity but no significant difference in ascorbic acid equivalent antioxidant capacity (AEAC) between dose 500 µg/mL (5.00 ± 0.35 AEAC) and 1000 µg/ mL (5.70 ± 0.29 AEAC) extract. GC-MS analysis showed the extract contained 88.93% of fatty acids and terpenoids, especially n-hexadecanoic acid, spathulenol, and phytol. MTT assay showed no IC50 value for the tested extract dose on UVB-induced HaCaT. Thus, the results suggest the potential application of hexane extract of C. odontophyllum Miq. leaf in terpenoids’ studies. In-depth research and isolation of compounds of interest should be done to develop it as a viable medical phytotherapeutic agent. Penerbit Universiti Kebangsaan Malaysia 2022 Article PeerReviewed application/pdf en http://journalarticle.ukm.my/20742/1/079-087%2BMAB%2B2377.pdf Muhammad Wahizul Haswan Abdul Aziz, and Dayang Fredalina Basri, and Siti Fathiah Masre, and Ahmad Rohi Ghazali, (2022) Fatty acids and terpenoids from Canarium odontophyllum MIQ. Leaf and their antioxidant and cytotoxic effects on UVB-induced immortalized human keratinocytes cells (HACAT). Malaysian Applied Biology, 51 (3). pp. 79-87. ISSN 0126-8643 https://jms.mabjournal.com/index.php/mab/issue/view/46 |
| spellingShingle | Muhammad Wahizul Haswan Abdul Aziz, Dayang Fredalina Basri, Siti Fathiah Masre, Ahmad Rohi Ghazali, Fatty acids and terpenoids from Canarium odontophyllum MIQ. Leaf and their antioxidant and cytotoxic effects on UVB-induced immortalized human keratinocytes cells (HACAT) |
| title | Fatty acids and terpenoids from Canarium odontophyllum MIQ. Leaf and their antioxidant and cytotoxic effects on UVB-induced immortalized human keratinocytes cells (HACAT) |
| title_full | Fatty acids and terpenoids from Canarium odontophyllum MIQ. Leaf and their antioxidant and cytotoxic effects on UVB-induced immortalized human keratinocytes cells (HACAT) |
| title_fullStr | Fatty acids and terpenoids from Canarium odontophyllum MIQ. Leaf and their antioxidant and cytotoxic effects on UVB-induced immortalized human keratinocytes cells (HACAT) |
| title_full_unstemmed | Fatty acids and terpenoids from Canarium odontophyllum MIQ. Leaf and their antioxidant and cytotoxic effects on UVB-induced immortalized human keratinocytes cells (HACAT) |
| title_short | Fatty acids and terpenoids from Canarium odontophyllum MIQ. Leaf and their antioxidant and cytotoxic effects on UVB-induced immortalized human keratinocytes cells (HACAT) |
| title_sort | fatty acids and terpenoids from canarium odontophyllum miq. leaf and their antioxidant and cytotoxic effects on uvb-induced immortalized human keratinocytes cells (hacat) |
| url | http://journalarticle.ukm.my/20742/1/079-087%2BMAB%2B2377.pdf http://journalarticle.ukm.my/20742/ https://jms.mabjournal.com/index.php/mab/issue/view/46 |
| url_provider | http://journalarticle.ukm.my/ |