Synergism activity between Moringa oleifera and Azadirachta indica crude extract against Nilaparvata lugens (Hemiptera: Delphacidae)

Extract seeds of Moringa oleifera and Azadirachta indica were tested separately and in mixture (1:9, 2:8, 3:7, 4:6, 5:5, 6:4, 7:3, 8:2, 9:1) in the laboratory for their insecticidal activity against Nilaparvata lugens (Hemiptera: Delphacidae). Moringa oleifera and A. indica plant materials were e...

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Main Authors:	Muhammad Nabil Fariq Mohd Mohkheri, Masdah Mawi, Insyirah Ishak, Norhayu Asib
Format:	Article
Language:	en
Published:	Pusat Sistematik Serangga, Universiti Kebangsaan Malaysia 2021
Online Access:	http://journalarticle.ukm.my/18983/1/47049-173395-1-PB.pdf http://journalarticle.ukm.my/18983/ https://ejournal.ukm.my/serangga/issue/view/1448/showToc
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_version_	1834433573642305536
author	Muhammad Nabil Fariq Mohd Mohkheri, Masdah Mawi, Insyirah Ishak, Norhayu Asib,
author_facet	Muhammad Nabil Fariq Mohd Mohkheri, Masdah Mawi, Insyirah Ishak, Norhayu Asib,
author_sort	Muhammad Nabil Fariq Mohd Mohkheri,
building	Tun Sri Lanang Library
collection	Institutional Repository
content_provider	Universiti Kebangsaan Malaysia
content_source	UKM Journal Article Repository
continent	Asia
country	Malaysia
description	Extract seeds of Moringa oleifera and Azadirachta indica were tested separately and in mixture (1:9, 2:8, 3:7, 4:6, 5:5, 6:4, 7:3, 8:2, 9:1) in the laboratory for their insecticidal activity against Nilaparvata lugens (Hemiptera: Delphacidae). Moringa oleifera and A. indica plant materials were extracted with hexane using maceration technique. Insecticidal bioassays were done by leaf-dip bioassays method. Second to fourth instar nymph of N. lugens were fed extract treated paddy seedling and were observed for 24, 48 and 72 hours. The data were analyzed using PoloPlus V.2 program, analysis of variance (ANOVA) and CompuSyn software. The results showed that the yield of extraction for M. oleifera was 26.7%, while for A. indica was 3.0%. Based on LC50 value, mixture extract (3:7) of M. oleifera and A. indica (LC50=52.24 mg/L) were more toxic compared to other mixture ratios and single crude of M. oleifera (98.03mg/L) and A. indica (87.45mg/L). Based on mortality percentages at 72 hours, 0.05% mixture of M. oleifera and A. indica showed good efficacy against nymph of N. lugens with 74.81% compared to 0.025% and 0.01% mixture of M. oleifera and A. indica at 68.15% and 49.63%, respectively. Based on the combination index (CI), all mixtures have synergistic joint action against N. lugens nymph except ratio 8:2 (C1=0). For ratio 1:9, 2:8, 3:7, 4:6, 5:5 6:4, 7:3 and 9:1 the CI were 0.0048, 0.0086, 0.0091, 0.019, 0.0133, 0.047, 0.0163 and 0.0379 respectively.
format	Article
id	my-ukm.journal.18983
institution	Universiti Kebangsaan Malaysia
language	en
publishDate	2021
publisher	Pusat Sistematik Serangga, Universiti Kebangsaan Malaysia
record_format	eprints
spelling	my-ukm.journal.189832022-07-20T06:18:16Z http://journalarticle.ukm.my/18983/ Synergism activity between Moringa oleifera and Azadirachta indica crude extract against Nilaparvata lugens (Hemiptera: Delphacidae) Muhammad Nabil Fariq Mohd Mohkheri, Masdah Mawi, Insyirah Ishak, Norhayu Asib, Extract seeds of Moringa oleifera and Azadirachta indica were tested separately and in mixture (1:9, 2:8, 3:7, 4:6, 5:5, 6:4, 7:3, 8:2, 9:1) in the laboratory for their insecticidal activity against Nilaparvata lugens (Hemiptera: Delphacidae). Moringa oleifera and A. indica plant materials were extracted with hexane using maceration technique. Insecticidal bioassays were done by leaf-dip bioassays method. Second to fourth instar nymph of N. lugens were fed extract treated paddy seedling and were observed for 24, 48 and 72 hours. The data were analyzed using PoloPlus V.2 program, analysis of variance (ANOVA) and CompuSyn software. The results showed that the yield of extraction for M. oleifera was 26.7%, while for A. indica was 3.0%. Based on LC50 value, mixture extract (3:7) of M. oleifera and A. indica (LC50=52.24 mg/L) were more toxic compared to other mixture ratios and single crude of M. oleifera (98.03mg/L) and A. indica (87.45mg/L). Based on mortality percentages at 72 hours, 0.05% mixture of M. oleifera and A. indica showed good efficacy against nymph of N. lugens with 74.81% compared to 0.025% and 0.01% mixture of M. oleifera and A. indica at 68.15% and 49.63%, respectively. Based on the combination index (CI), all mixtures have synergistic joint action against N. lugens nymph except ratio 8:2 (C1=0). For ratio 1:9, 2:8, 3:7, 4:6, 5:5 6:4, 7:3 and 9:1 the CI were 0.0048, 0.0086, 0.0091, 0.019, 0.0133, 0.047, 0.0163 and 0.0379 respectively. Pusat Sistematik Serangga, Universiti Kebangsaan Malaysia 2021 Article PeerReviewed application/pdf en http://journalarticle.ukm.my/18983/1/47049-173395-1-PB.pdf Muhammad Nabil Fariq Mohd Mohkheri, and Masdah Mawi, and Insyirah Ishak, and Norhayu Asib, (2021) Synergism activity between Moringa oleifera and Azadirachta indica crude extract against Nilaparvata lugens (Hemiptera: Delphacidae). Serangga, 26 (4). pp. 72-83. ISSN 1394-5130 https://ejournal.ukm.my/serangga/issue/view/1448/showToc
spellingShingle	Muhammad Nabil Fariq Mohd Mohkheri, Masdah Mawi, Insyirah Ishak, Norhayu Asib, Synergism activity between Moringa oleifera and Azadirachta indica crude extract against Nilaparvata lugens (Hemiptera: Delphacidae)
title	Synergism activity between Moringa oleifera and Azadirachta indica crude extract against Nilaparvata lugens (Hemiptera: Delphacidae)
title_full	Synergism activity between Moringa oleifera and Azadirachta indica crude extract against Nilaparvata lugens (Hemiptera: Delphacidae)
title_fullStr	Synergism activity between Moringa oleifera and Azadirachta indica crude extract against Nilaparvata lugens (Hemiptera: Delphacidae)
title_full_unstemmed	Synergism activity between Moringa oleifera and Azadirachta indica crude extract against Nilaparvata lugens (Hemiptera: Delphacidae)
title_short	Synergism activity between Moringa oleifera and Azadirachta indica crude extract against Nilaparvata lugens (Hemiptera: Delphacidae)
title_sort	synergism activity between moringa oleifera and azadirachta indica crude extract against nilaparvata lugens (hemiptera: delphacidae)
url	http://journalarticle.ukm.my/18983/1/47049-173395-1-PB.pdf http://journalarticle.ukm.my/18983/ https://ejournal.ukm.my/serangga/issue/view/1448/showToc
url_provider	http://journalarticle.ukm.my/

Synergism activity between Moringa oleifera and Azadirachta indica crude extract against Nilaparvata lugens (Hemiptera: Delphacidae)

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