Evaluation of Trichoderma asperellum B1902 in controlling Fusarium wilt of cavendish banana cultivar
Trichoderma species is one of the microorganisms with antagonistic properties as biological control agents. In the banana industry, Fusarium wilt disease caused by Fusarium oxysporum f. sp. cubense (Foc) has been practically managed using chemical pesticides that led to environmental disruptions, in...
Saved in:
| Main Authors: | , , |
|---|---|
| Format: | Article |
| Language: | en |
| Published: |
Penerbit Universiti Kebangsaan Malaysia
2021
|
| Online Access: | http://journalarticle.ukm.my/18054/1/5.pdf http://journalarticle.ukm.my/18054/ https://www.ukm.my/jsm/malay_journals/jilid50bil9_2021/KandunganJilid50Bil9_2021.html |
| Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
| _version_ | 1834433332577828864 |
|---|---|
| author | Sharifah Siti Maryam Syd Abdul Rahman, Nur Ain Izzati Mohd Zainudin, Nor Azwady Abd. Aziz, |
| author_facet | Sharifah Siti Maryam Syd Abdul Rahman, Nur Ain Izzati Mohd Zainudin, Nor Azwady Abd. Aziz, |
| author_sort | Sharifah Siti Maryam Syd Abdul Rahman, |
| building | Tun Sri Lanang Library |
| collection | Institutional Repository |
| content_provider | Universiti Kebangsaan Malaysia |
| content_source | UKM Journal Article Repository |
| continent | Asia |
| country | Malaysia |
| description | Trichoderma species is one of the microorganisms with antagonistic properties as biological control agents. In the banana industry, Fusarium wilt disease caused by Fusarium oxysporum f. sp. cubense (Foc) has been practically managed using chemical pesticides that led to environmental disruptions, ineffective conditions and disease resistance. In preliminary study, T. asperellum gave better result compared to other species in inhibiting the growth of Foc in in vitro condition. Therefore, the aim of this study was to examine the effects of T. asperellum as a biological control of Fusarium wilt disease of banana. A total of 326 fungal isolates were isolated from soil samples obtained around Malaysia and identified as Trichoderma species based on phenotype characteristics. The species identity for the best candidates from dual culture test was confirmed based on internal transcribed spacers (ITS) and translation elongation factor 1 alpha (TEF-1α) sequence identity. In dual culture test, findings showed that three isolates with a high percentage inhibition of radial growth (PIRG) were observed in plates of T. asperellum isolates B1902 (84.85%), T2007 (77.78%) and C1667 (75.76%), which successfully inhibited the growth of F. oxysporum f. sp. cubense isolate 9888. Based on in vivo test, the best candidate was T. asperellum B1902 with lower disease severity index (DSI) value of 0.2 compared to the inoculated control with DSI of 3.6. As a conclusion, T. asperellum B1902 can be used as an alternative treatment in managing Fusarium wilt disease. Hence, future study should be focused on applying T. asperellum as a biocontrol agent in the field and controlling other plant diseases in the agricultural plantation. |
| format | Article |
| id | my-ukm.journal.18054 |
| institution | Universiti Kebangsaan Malaysia |
| language | en |
| publishDate | 2021 |
| publisher | Penerbit Universiti Kebangsaan Malaysia |
| record_format | eprints |
| spelling | my-ukm.journal.180542022-02-18T00:39:27Z http://journalarticle.ukm.my/18054/ Evaluation of Trichoderma asperellum B1902 in controlling Fusarium wilt of cavendish banana cultivar Sharifah Siti Maryam Syd Abdul Rahman, Nur Ain Izzati Mohd Zainudin, Nor Azwady Abd. Aziz, Trichoderma species is one of the microorganisms with antagonistic properties as biological control agents. In the banana industry, Fusarium wilt disease caused by Fusarium oxysporum f. sp. cubense (Foc) has been practically managed using chemical pesticides that led to environmental disruptions, ineffective conditions and disease resistance. In preliminary study, T. asperellum gave better result compared to other species in inhibiting the growth of Foc in in vitro condition. Therefore, the aim of this study was to examine the effects of T. asperellum as a biological control of Fusarium wilt disease of banana. A total of 326 fungal isolates were isolated from soil samples obtained around Malaysia and identified as Trichoderma species based on phenotype characteristics. The species identity for the best candidates from dual culture test was confirmed based on internal transcribed spacers (ITS) and translation elongation factor 1 alpha (TEF-1α) sequence identity. In dual culture test, findings showed that three isolates with a high percentage inhibition of radial growth (PIRG) were observed in plates of T. asperellum isolates B1902 (84.85%), T2007 (77.78%) and C1667 (75.76%), which successfully inhibited the growth of F. oxysporum f. sp. cubense isolate 9888. Based on in vivo test, the best candidate was T. asperellum B1902 with lower disease severity index (DSI) value of 0.2 compared to the inoculated control with DSI of 3.6. As a conclusion, T. asperellum B1902 can be used as an alternative treatment in managing Fusarium wilt disease. Hence, future study should be focused on applying T. asperellum as a biocontrol agent in the field and controlling other plant diseases in the agricultural plantation. Penerbit Universiti Kebangsaan Malaysia 2021-09 Article PeerReviewed application/pdf en http://journalarticle.ukm.my/18054/1/5.pdf Sharifah Siti Maryam Syd Abdul Rahman, and Nur Ain Izzati Mohd Zainudin, and Nor Azwady Abd. Aziz, (2021) Evaluation of Trichoderma asperellum B1902 in controlling Fusarium wilt of cavendish banana cultivar. Sains Malaysiana, 50 (9). pp. 2549-2561. ISSN 0126-6039 https://www.ukm.my/jsm/malay_journals/jilid50bil9_2021/KandunganJilid50Bil9_2021.html |
| spellingShingle | Sharifah Siti Maryam Syd Abdul Rahman, Nur Ain Izzati Mohd Zainudin, Nor Azwady Abd. Aziz, Evaluation of Trichoderma asperellum B1902 in controlling Fusarium wilt of cavendish banana cultivar |
| title | Evaluation of Trichoderma asperellum B1902 in controlling Fusarium wilt of cavendish banana cultivar |
| title_full | Evaluation of Trichoderma asperellum B1902 in controlling Fusarium wilt of cavendish banana cultivar |
| title_fullStr | Evaluation of Trichoderma asperellum B1902 in controlling Fusarium wilt of cavendish banana cultivar |
| title_full_unstemmed | Evaluation of Trichoderma asperellum B1902 in controlling Fusarium wilt of cavendish banana cultivar |
| title_short | Evaluation of Trichoderma asperellum B1902 in controlling Fusarium wilt of cavendish banana cultivar |
| title_sort | evaluation of trichoderma asperellum b1902 in controlling fusarium wilt of cavendish banana cultivar |
| url | http://journalarticle.ukm.my/18054/1/5.pdf http://journalarticle.ukm.my/18054/ https://www.ukm.my/jsm/malay_journals/jilid50bil9_2021/KandunganJilid50Bil9_2021.html |
| url_provider | http://journalarticle.ukm.my/ |