Effects of light illumination and subculture frequency on biomass production in cell suspension cultures of Clinacanthus nutans
Clinacanthus nutans (Burm. f.) Lindau is a medicinal plant rich in polyphenols and possesses multiple pharmacological properties. It is widely used as a side treatment for cancer and to prevent oxidative stress-related diseases. The present study aims to investigate the effects of light illuminati...
Saved in:
| Main Authors: | , , |
|---|---|
| Format: | Article |
| Language: | en |
| Published: |
Penerbit Universiti Kebangsaan Malaysia
2021
|
| Online Access: | http://journalarticle.ukm.my/17907/1/197-204%2B%2B%2BBee%2BLynn%2BChew.pdf http://journalarticle.ukm.my/17907/ https://jms.mabjournal.com/index.php/mab/issue/view/2 |
| Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
| _version_ | 1834433293840285696 |
|---|---|
| author | Bong, Fui Joo Sreeramanan Subramaniam, Chew, Bee Lynn |
| author_facet | Bong, Fui Joo Sreeramanan Subramaniam, Chew, Bee Lynn |
| author_sort | Bong, Fui Joo |
| building | Tun Sri Lanang Library |
| collection | Institutional Repository |
| content_provider | Universiti Kebangsaan Malaysia |
| content_source | UKM Journal Article Repository |
| continent | Asia |
| country | Malaysia |
| description | Clinacanthus nutans (Burm. f.) Lindau is a medicinal plant rich in polyphenols and possesses multiple pharmacological
properties. It is widely used as a side treatment for cancer and to prevent oxidative stress-related diseases. The present
study aims to investigate the effects of light illumination and subculture frequency on the growth and proliferation of cells
in the suspension culture of C. nutans. Friable callus induced from leaf explants C. nutans were cultured in liquid Murashige
& Skoog medium supplemented with 0.25 mg/L 2, 4-dichlorophenoxyacetic acid, and 0.25 mg/L 6-benzylaminopurine to
initiate the cell suspension culture. The suspension cultures were incubated under different conditions (16 hr photoperiod
and continuous darkness). Subcultures were conducted every two weeks for ten subculture cycles. Results revealed that
higher cell biomass was obtained under the exposure of 16 hr photoperiod with the highest biomass production at the fifth
subculture cycle (growth index of 11.8) exhibiting stable growth from the eighth to the tenth subculture cycle. The suspension
cells showed stable growth rates up to the 10th subculture cycle producing small aggregates and single cells. The present
study indicated that light illumination significantly impacts the growth of suspension cultures for C. nutans in the production
of valuable secondary metabolites. |
| format | Article |
| id | my-ukm.journal.17907 |
| institution | Universiti Kebangsaan Malaysia |
| language | en |
| publishDate | 2021 |
| publisher | Penerbit Universiti Kebangsaan Malaysia |
| record_format | eprints |
| spelling | my-ukm.journal.179072022-01-13T01:31:50Z http://journalarticle.ukm.my/17907/ Effects of light illumination and subculture frequency on biomass production in cell suspension cultures of Clinacanthus nutans Bong, Fui Joo Sreeramanan Subramaniam, Chew, Bee Lynn Clinacanthus nutans (Burm. f.) Lindau is a medicinal plant rich in polyphenols and possesses multiple pharmacological properties. It is widely used as a side treatment for cancer and to prevent oxidative stress-related diseases. The present study aims to investigate the effects of light illumination and subculture frequency on the growth and proliferation of cells in the suspension culture of C. nutans. Friable callus induced from leaf explants C. nutans were cultured in liquid Murashige & Skoog medium supplemented with 0.25 mg/L 2, 4-dichlorophenoxyacetic acid, and 0.25 mg/L 6-benzylaminopurine to initiate the cell suspension culture. The suspension cultures were incubated under different conditions (16 hr photoperiod and continuous darkness). Subcultures were conducted every two weeks for ten subculture cycles. Results revealed that higher cell biomass was obtained under the exposure of 16 hr photoperiod with the highest biomass production at the fifth subculture cycle (growth index of 11.8) exhibiting stable growth from the eighth to the tenth subculture cycle. The suspension cells showed stable growth rates up to the 10th subculture cycle producing small aggregates and single cells. The present study indicated that light illumination significantly impacts the growth of suspension cultures for C. nutans in the production of valuable secondary metabolites. Penerbit Universiti Kebangsaan Malaysia 2021 Article PeerReviewed application/pdf en http://journalarticle.ukm.my/17907/1/197-204%2B%2B%2BBee%2BLynn%2BChew.pdf Bong, Fui Joo and Sreeramanan Subramaniam, and Chew, Bee Lynn (2021) Effects of light illumination and subculture frequency on biomass production in cell suspension cultures of Clinacanthus nutans. Malaysian Applied Biology, 50 (1). pp. 197-204. ISSN 0126-8643 https://jms.mabjournal.com/index.php/mab/issue/view/2 |
| spellingShingle | Bong, Fui Joo Sreeramanan Subramaniam, Chew, Bee Lynn Effects of light illumination and subculture frequency on biomass production in cell suspension cultures of Clinacanthus nutans |
| title | Effects of light illumination and subculture frequency on biomass production in cell suspension cultures of Clinacanthus nutans |
| title_full | Effects of light illumination and subculture frequency on biomass production in cell suspension cultures of Clinacanthus nutans |
| title_fullStr | Effects of light illumination and subculture frequency on biomass production in cell suspension cultures of Clinacanthus nutans |
| title_full_unstemmed | Effects of light illumination and subculture frequency on biomass production in cell suspension cultures of Clinacanthus nutans |
| title_short | Effects of light illumination and subculture frequency on biomass production in cell suspension cultures of Clinacanthus nutans |
| title_sort | effects of light illumination and subculture frequency on biomass production in cell suspension cultures of clinacanthus nutans |
| url | http://journalarticle.ukm.my/17907/1/197-204%2B%2B%2BBee%2BLynn%2BChew.pdf http://journalarticle.ukm.my/17907/ https://jms.mabjournal.com/index.php/mab/issue/view/2 |
| url_provider | http://journalarticle.ukm.my/ |