Encapsulation of miRNA in chitosan nanoparticles as a candidate for an anti-metastatic agent in cancer therapy
MicroRNAs (miRNA) have been utilised as a repressor molecule for metastasis of tumours, as it inhibits fundamental processes related to cellular and physiological pathway of the tumour at the mRNA level. However, therapeutic application of miRNAs is impaired by premature degradation in the extrace...
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| Format: | Article |
| Language: | en |
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Penerbit Universiti Kebangsaan Malaysia
2017
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| Online Access: | http://journalarticle.ukm.my/12317/1/46_01_24.pdf http://journalarticle.ukm.my/12317/ http://www.mabjournal.com/index.php?option=com_content&view=article&id=623&catid=59:current-view&Itemid=56 |
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| author | Syazaira Arham Yahya Ariff, Khatijah Yusoff, Mas Jaffri Masarudin, |
| author_facet | Syazaira Arham Yahya Ariff, Khatijah Yusoff, Mas Jaffri Masarudin, |
| author_sort | Syazaira Arham Yahya Ariff, |
| building | Tun Sri Lanang Library |
| collection | Institutional Repository |
| content_provider | Universiti Kebangsaan Malaysia |
| content_source | UKM Journal Article Repository |
| continent | Asia |
| country | Malaysia |
| description | MicroRNAs (miRNA) have been utilised as a repressor molecule for metastasis of tumours, as it inhibits fundamental processes
related to cellular and physiological pathway of the tumour at the mRNA level. However, therapeutic application of miRNAs
is impaired by premature degradation in the extracellular environment by endonucleases. This research describes the
optimisation, chemical, and morphological characterisation of nanoparticles for effective encapsulation of miRNA-186 and
evaluate its efficiency as anti-metastatic agent in non-small cell lung carcinoma monolayer. Through ionic gelation methods,
the miRNA was encapsulated in chitosan nanoparticles (CNPs), a drug carrier with high particle stability, low cellular toxicity,
and robust preparation methods. Physiochemical and morphological characterization analysis through light scattering analysis
showed miRNA-CNP sizes below 200 nm, with a low polydispersity index and exhibition of irregular spherical shape of the
nanoparticles synthesised through FESEM analysis. Additionally, in vitro nanoparticle efficacy evaluated through scratch
assay suggests a decrease in invasion ability of cancer cells exhibited by miRNA-CNP. |
| format | Article |
| id | my-ukm.journal.12317 |
| institution | Universiti Kebangsaan Malaysia |
| language | en |
| publishDate | 2017 |
| publisher | Penerbit Universiti Kebangsaan Malaysia |
| record_format | eprints |
| spelling | my-ukm.journal.123172018-11-16T12:35:20Z http://journalarticle.ukm.my/12317/ Encapsulation of miRNA in chitosan nanoparticles as a candidate for an anti-metastatic agent in cancer therapy Syazaira Arham Yahya Ariff, Khatijah Yusoff, Mas Jaffri Masarudin, MicroRNAs (miRNA) have been utilised as a repressor molecule for metastasis of tumours, as it inhibits fundamental processes related to cellular and physiological pathway of the tumour at the mRNA level. However, therapeutic application of miRNAs is impaired by premature degradation in the extracellular environment by endonucleases. This research describes the optimisation, chemical, and morphological characterisation of nanoparticles for effective encapsulation of miRNA-186 and evaluate its efficiency as anti-metastatic agent in non-small cell lung carcinoma monolayer. Through ionic gelation methods, the miRNA was encapsulated in chitosan nanoparticles (CNPs), a drug carrier with high particle stability, low cellular toxicity, and robust preparation methods. Physiochemical and morphological characterization analysis through light scattering analysis showed miRNA-CNP sizes below 200 nm, with a low polydispersity index and exhibition of irregular spherical shape of the nanoparticles synthesised through FESEM analysis. Additionally, in vitro nanoparticle efficacy evaluated through scratch assay suggests a decrease in invasion ability of cancer cells exhibited by miRNA-CNP. Penerbit Universiti Kebangsaan Malaysia 2017-03 Article PeerReviewed application/pdf en http://journalarticle.ukm.my/12317/1/46_01_24.pdf Syazaira Arham Yahya Ariff, and Khatijah Yusoff, and Mas Jaffri Masarudin, (2017) Encapsulation of miRNA in chitosan nanoparticles as a candidate for an anti-metastatic agent in cancer therapy. Malaysian Applied Biology, 46 (1). pp. 165-170. ISSN 0126-8643 http://www.mabjournal.com/index.php?option=com_content&view=article&id=623&catid=59:current-view&Itemid=56 |
| spellingShingle | Syazaira Arham Yahya Ariff, Khatijah Yusoff, Mas Jaffri Masarudin, Encapsulation of miRNA in chitosan nanoparticles as a candidate for an anti-metastatic agent in cancer therapy |
| title | Encapsulation of miRNA in chitosan nanoparticles
as a candidate for an anti-metastatic agent
in cancer therapy |
| title_full | Encapsulation of miRNA in chitosan nanoparticles
as a candidate for an anti-metastatic agent
in cancer therapy |
| title_fullStr | Encapsulation of miRNA in chitosan nanoparticles
as a candidate for an anti-metastatic agent
in cancer therapy |
| title_full_unstemmed | Encapsulation of miRNA in chitosan nanoparticles
as a candidate for an anti-metastatic agent
in cancer therapy |
| title_short | Encapsulation of miRNA in chitosan nanoparticles
as a candidate for an anti-metastatic agent
in cancer therapy |
| title_sort | encapsulation of mirna in chitosan nanoparticles
as a candidate for an anti-metastatic agent
in cancer therapy |
| url | http://journalarticle.ukm.my/12317/1/46_01_24.pdf http://journalarticle.ukm.my/12317/ http://www.mabjournal.com/index.php?option=com_content&view=article&id=623&catid=59:current-view&Itemid=56 |
| url_provider | http://journalarticle.ukm.my/ |