Characterization of immunoglobulin E-binding proteins (IgE) of scomberomorus commerson lacepede
The objective of this study was to determine the Immunoglobulin E-binding proteins (IgE) and major allergens of Scomberomorus commerson Lacepede(Narrow-barred Spanish mackerel). Allergen extracts were obtained from uncooked and cooked fish by homogenization in phosphate-buffered saline followed by c...
Saved in:
| Main Authors: | , , , , |
|---|---|
| Format: | Article |
| Language: | en |
| Published: |
penerbit ukm
2005
|
| Online Access: | http://journalarticle.ukm.my/983/1/jurnal36.pdf http://journalarticle.ukm.my/983/ http://www.fskb.ukm.my/fskbold/jurnal |
| Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
| _version_ | 1831353110659858432 |
|---|---|
| author | Rosmilah Misnan, Shahnaz Murad, Masita Arip, Noormalin Abdullah, Jamaludin Mohamed, |
| author_facet | Rosmilah Misnan, Shahnaz Murad, Masita Arip, Noormalin Abdullah, Jamaludin Mohamed, |
| author_sort | Rosmilah Misnan, |
| building | Tun Sri Lanang Library |
| collection | Institutional Repository |
| content_provider | Universiti Kebangsaan Malaysia |
| content_source | UKM Journal Article Repository |
| continent | Asia |
| country | Malaysia |
| description | The objective of this study was to determine the Immunoglobulin E-binding proteins (IgE) and major allergens of Scomberomorus commerson Lacepede(Narrow-barred Spanish mackerel). Allergen extracts were obtained from uncooked and cooked fish by homogenization in phosphate-buffered saline followed by continuous extraction at 4oC or on ice. Protein profiles and IgEbinding patterns were then detected by means of sodium dodecyl polyacrylamidegel electrophoresis (SDS-PAGE) and immunoblotting using sera from patients sensitized to the fish. SDS-PAGE of the uncooked fish extracts revealed 26 protein bands in the range of about 11 to >175 kD, while the cooked extracts produced fewer protein bands. Immunoblotting demonstrated 17 IgE-binding bands, ranging in molecular weight from 11 to 151 kD. Two components with molecular weight of about ~50 and 42 kD showed the highest frequency of IgE-binding
(62.2 and 51.4% respectively) and were identified as the major allergens of this fish allergy. Other IgE-binding proteins including a protein at ~12 kD which was equivalent in size to parvalbumin were identified as the minor
allergens. |
| format | Article |
| id | my-ukm.journal-983 |
| institution | Universiti Kebangsaan Malaysia |
| language | en |
| publishDate | 2005 |
| publisher | penerbit ukm |
| record_format | eprints |
| spelling | my-ukm.journal-9832016-12-14T06:28:29Z http://journalarticle.ukm.my/983/ Characterization of immunoglobulin E-binding proteins (IgE) of scomberomorus commerson lacepede Rosmilah Misnan, Shahnaz Murad, Masita Arip, Noormalin Abdullah, Jamaludin Mohamed, The objective of this study was to determine the Immunoglobulin E-binding proteins (IgE) and major allergens of Scomberomorus commerson Lacepede(Narrow-barred Spanish mackerel). Allergen extracts were obtained from uncooked and cooked fish by homogenization in phosphate-buffered saline followed by continuous extraction at 4oC or on ice. Protein profiles and IgEbinding patterns were then detected by means of sodium dodecyl polyacrylamidegel electrophoresis (SDS-PAGE) and immunoblotting using sera from patients sensitized to the fish. SDS-PAGE of the uncooked fish extracts revealed 26 protein bands in the range of about 11 to >175 kD, while the cooked extracts produced fewer protein bands. Immunoblotting demonstrated 17 IgE-binding bands, ranging in molecular weight from 11 to 151 kD. Two components with molecular weight of about ~50 and 42 kD showed the highest frequency of IgE-binding (62.2 and 51.4% respectively) and were identified as the major allergens of this fish allergy. Other IgE-binding proteins including a protein at ~12 kD which was equivalent in size to parvalbumin were identified as the minor allergens. penerbit ukm 2005 Article PeerReviewed application/pdf en http://journalarticle.ukm.my/983/1/jurnal36.pdf Rosmilah Misnan, and Shahnaz Murad, and Masita Arip, and Noormalin Abdullah, and Jamaludin Mohamed, (2005) Characterization of immunoglobulin E-binding proteins (IgE) of scomberomorus commerson lacepede. Jurnal Sains Kesihatan Malaysia, 3 (2). pp. 79-87. ISSN 1675-8161 http://www.fskb.ukm.my/fskbold/jurnal |
| spellingShingle | Rosmilah Misnan, Shahnaz Murad, Masita Arip, Noormalin Abdullah, Jamaludin Mohamed, Characterization of immunoglobulin E-binding proteins (IgE) of scomberomorus commerson lacepede |
| title | Characterization of immunoglobulin E-binding proteins (IgE) of scomberomorus commerson lacepede |
| title_full | Characterization of immunoglobulin E-binding proteins (IgE) of scomberomorus commerson lacepede |
| title_fullStr | Characterization of immunoglobulin E-binding proteins (IgE) of scomberomorus commerson lacepede |
| title_full_unstemmed | Characterization of immunoglobulin E-binding proteins (IgE) of scomberomorus commerson lacepede |
| title_short | Characterization of immunoglobulin E-binding proteins (IgE) of scomberomorus commerson lacepede |
| title_sort | characterization of immunoglobulin e-binding proteins (ige) of scomberomorus commerson lacepede |
| url | http://journalarticle.ukm.my/983/1/jurnal36.pdf http://journalarticle.ukm.my/983/ http://www.fskb.ukm.my/fskbold/jurnal |
| url_provider | http://journalarticle.ukm.my/ |