Effect of preparation and extraction parameters of Banana (Musa balbisiana cv. Saba) inflorescence on their antibacterial activities
The study aimed to determine the influence of sample preparation and extraction parameters on the antibacterial activity of inflorescences from banana (Musa balbisiaba cv. Saba). Banana inflorescences were extracted using various solvent extractions and tested for antibacterial activity using agar-w...
Saved in:
| Main Authors: | , , , , |
|---|---|
| Format: | Article |
| Language: | en |
| Published: |
Universiti Kebangsaan Malaysia
2015
|
| Online Access: | http://journalarticle.ukm.my/9265/1/12_Hoe_Seng_Tin.pdf http://journalarticle.ukm.my/9265/ http://www.ukm.my/jsm/malay_journals/jilid44bil9_2015/KandunganJilid44Bil9_2015.html |
| Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
| _version_ | 1831356708036804608 |
|---|---|
| author | Hoe, Seng Tin Birdie Scott Padam, Charles S. Vairappan, Mohd Ismail Abdullah, Fook, Yee Chye |
| author_facet | Hoe, Seng Tin Birdie Scott Padam, Charles S. Vairappan, Mohd Ismail Abdullah, Fook, Yee Chye |
| author_sort | Hoe, Seng Tin |
| building | Tun Sri Lanang Library |
| collection | Institutional Repository |
| content_provider | Universiti Kebangsaan Malaysia |
| content_source | UKM Journal Article Repository |
| continent | Asia |
| country | Malaysia |
| description | The study aimed to determine the influence of sample preparation and extraction parameters on the antibacterial activity of inflorescences from banana (Musa balbisiaba cv. Saba). Banana inflorescences were extracted using various solvent extractions and tested for antibacterial activity using agar-well diffusion assay against gram-positive bacteria (Staphylococcus aureus, Bacillus cereus, Listeria monocytogenes and Brochothrix thermosphacta) and gram-negative bacteria (Salmonella typhimurium, Salmonella enteritidis, Escherichia coli O157:H7, Enterobacter sakazakii, Yersinia enterocolitica and Vibrio parahaemolyticus) The effects of geographical origin, drying methods and extraction parameters (sample-to-solvent ratio, extraction time and temperature as well as methanol to solvent ratio) on antibacterial activity of the banana by-product were carried out. Among all the extracts evaluated, methanolic extract from the buds showed significant higher inhibitory against all gram positive bacteria ranging from 12.56-13.54 mm. Interestingly, no significant different (p>0.05) was observed on the effect of geographical origin as well as extraction methods on the antibacterial capacity. Meanwhile, the extracts produced from 50°C oven dried sample seem to have comparable antibacterial activity with the freeze dried samples. Extraction parameters (sample-to-solvent ratio, extraction time and temperature as well as methanol to solvent ratio) were found responsible in determining the efficacy of the antibacterial. In conclusion, methanolic extracts from banana inflorescence buds could be a new source of natural antibacterial and further bioassay guided fractionation should be carried out to determine the bioactive compounds and their biological activities. |
| format | Article |
| id | my-ukm.journal-9265 |
| institution | Universiti Kebangsaan Malaysia |
| language | en |
| publishDate | 2015 |
| publisher | Universiti Kebangsaan Malaysia |
| record_format | eprints |
| spelling | my-ukm.journal-92652016-12-14T06:49:24Z http://journalarticle.ukm.my/9265/ Effect of preparation and extraction parameters of Banana (Musa balbisiana cv. Saba) inflorescence on their antibacterial activities Hoe, Seng Tin Birdie Scott Padam, Charles S. Vairappan, Mohd Ismail Abdullah, Fook, Yee Chye The study aimed to determine the influence of sample preparation and extraction parameters on the antibacterial activity of inflorescences from banana (Musa balbisiaba cv. Saba). Banana inflorescences were extracted using various solvent extractions and tested for antibacterial activity using agar-well diffusion assay against gram-positive bacteria (Staphylococcus aureus, Bacillus cereus, Listeria monocytogenes and Brochothrix thermosphacta) and gram-negative bacteria (Salmonella typhimurium, Salmonella enteritidis, Escherichia coli O157:H7, Enterobacter sakazakii, Yersinia enterocolitica and Vibrio parahaemolyticus) The effects of geographical origin, drying methods and extraction parameters (sample-to-solvent ratio, extraction time and temperature as well as methanol to solvent ratio) on antibacterial activity of the banana by-product were carried out. Among all the extracts evaluated, methanolic extract from the buds showed significant higher inhibitory against all gram positive bacteria ranging from 12.56-13.54 mm. Interestingly, no significant different (p>0.05) was observed on the effect of geographical origin as well as extraction methods on the antibacterial capacity. Meanwhile, the extracts produced from 50°C oven dried sample seem to have comparable antibacterial activity with the freeze dried samples. Extraction parameters (sample-to-solvent ratio, extraction time and temperature as well as methanol to solvent ratio) were found responsible in determining the efficacy of the antibacterial. In conclusion, methanolic extracts from banana inflorescence buds could be a new source of natural antibacterial and further bioassay guided fractionation should be carried out to determine the bioactive compounds and their biological activities. Universiti Kebangsaan Malaysia 2015-09 Article PeerReviewed application/pdf en http://journalarticle.ukm.my/9265/1/12_Hoe_Seng_Tin.pdf Hoe, Seng Tin and Birdie Scott Padam, and Charles S. Vairappan, and Mohd Ismail Abdullah, and Fook, Yee Chye (2015) Effect of preparation and extraction parameters of Banana (Musa balbisiana cv. Saba) inflorescence on their antibacterial activities. Sains Malaysiana, 44 (9). pp. 1301-1307. ISSN 0126-6039 http://www.ukm.my/jsm/malay_journals/jilid44bil9_2015/KandunganJilid44Bil9_2015.html |
| spellingShingle | Hoe, Seng Tin Birdie Scott Padam, Charles S. Vairappan, Mohd Ismail Abdullah, Fook, Yee Chye Effect of preparation and extraction parameters of Banana (Musa balbisiana cv. Saba) inflorescence on their antibacterial activities |
| title | Effect of preparation and extraction parameters of Banana (Musa balbisiana cv. Saba) inflorescence on their antibacterial activities |
| title_full | Effect of preparation and extraction parameters of Banana (Musa balbisiana cv. Saba) inflorescence on their antibacterial activities |
| title_fullStr | Effect of preparation and extraction parameters of Banana (Musa balbisiana cv. Saba) inflorescence on their antibacterial activities |
| title_full_unstemmed | Effect of preparation and extraction parameters of Banana (Musa balbisiana cv. Saba) inflorescence on their antibacterial activities |
| title_short | Effect of preparation and extraction parameters of Banana (Musa balbisiana cv. Saba) inflorescence on their antibacterial activities |
| title_sort | effect of preparation and extraction parameters of banana (musa balbisiana cv. saba) inflorescence on their antibacterial activities |
| url | http://journalarticle.ukm.my/9265/1/12_Hoe_Seng_Tin.pdf http://journalarticle.ukm.my/9265/ http://www.ukm.my/jsm/malay_journals/jilid44bil9_2015/KandunganJilid44Bil9_2015.html |
| url_provider | http://journalarticle.ukm.my/ |