Molecular characterisation of sago starch using gel permeation chromatography multi-angle laser light scattering
The molecular characteristics of sago starch (native and debranched) were determined using a gel permeation chromatograpy multi-angle laser light scattering (GPC-MALLS) method. The method involves the optimisation of sample solubilisation and GPC operating conditions. The weight-average molecular we...
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Universiti Kebangsaan Malaysia
2010
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| Online Access: | http://journalarticle.ukm.my/7441/1/26_Ayiesah.pdf http://journalarticle.ukm.my/7441/ http://www.ukm.my/jsm/english_journals/vol39num6_2010/contentsVol39num6_2010.html |
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| author | Zainon Othman, Saphwan Al-Assaf, Osman Hassan, |
| author_facet | Zainon Othman, Saphwan Al-Assaf, Osman Hassan, |
| author_sort | Zainon Othman, |
| building | Tun Sri Lanang Library |
| collection | Institutional Repository |
| content_provider | Universiti Kebangsaan Malaysia |
| content_source | UKM Journal Article Repository |
| continent | Asia |
| country | Malaysia |
| description | The molecular characteristics of sago starch (native and debranched) were determined using a gel permeation chromatograpy multi-angle laser light scattering (GPC-MALLS) method. The method involves the optimisation of sample solubilisation and GPC operating conditions. The weight-average molecular weight Mw of native and debranched sago starch determined was 29.1 ± 2.1 °— 106 and 1.87 ± 0.4 °— 105 gmol-1 respectively while radius of gyration Rg was 123.6 and 59.3 nm respectively. The reduction in Mw and Rg in debranched sago was attributed to the hydrolysis of α-1,6 glycosidic linkages by pullulanase to smaller oligosaccharides. |
| format | Article |
| id | my-ukm.journal-7441 |
| institution | Universiti Kebangsaan Malaysia |
| language | en |
| publishDate | 2010 |
| publisher | Universiti Kebangsaan Malaysia |
| record_format | eprints |
| spelling | my-ukm.journal-74412016-12-14T06:44:06Z http://journalarticle.ukm.my/7441/ Molecular characterisation of sago starch using gel permeation chromatography multi-angle laser light scattering Zainon Othman, Saphwan Al-Assaf, Osman Hassan, The molecular characteristics of sago starch (native and debranched) were determined using a gel permeation chromatograpy multi-angle laser light scattering (GPC-MALLS) method. The method involves the optimisation of sample solubilisation and GPC operating conditions. The weight-average molecular weight Mw of native and debranched sago starch determined was 29.1 ± 2.1 °— 106 and 1.87 ± 0.4 °— 105 gmol-1 respectively while radius of gyration Rg was 123.6 and 59.3 nm respectively. The reduction in Mw and Rg in debranched sago was attributed to the hydrolysis of α-1,6 glycosidic linkages by pullulanase to smaller oligosaccharides. Universiti Kebangsaan Malaysia 2010-12 Article PeerReviewed application/pdf en http://journalarticle.ukm.my/7441/1/26_Ayiesah.pdf Zainon Othman, and Saphwan Al-Assaf, and Osman Hassan, (2010) Molecular characterisation of sago starch using gel permeation chromatography multi-angle laser light scattering. Sains Malaysiana, 39 (6). pp. 969-973. ISSN 0126-6039 http://www.ukm.my/jsm/english_journals/vol39num6_2010/contentsVol39num6_2010.html |
| spellingShingle | Zainon Othman, Saphwan Al-Assaf, Osman Hassan, Molecular characterisation of sago starch using gel permeation chromatography multi-angle laser light scattering |
| title | Molecular characterisation of sago starch using gel permeation chromatography multi-angle laser light scattering |
| title_full | Molecular characterisation of sago starch using gel permeation chromatography multi-angle laser light scattering |
| title_fullStr | Molecular characterisation of sago starch using gel permeation chromatography multi-angle laser light scattering |
| title_full_unstemmed | Molecular characterisation of sago starch using gel permeation chromatography multi-angle laser light scattering |
| title_short | Molecular characterisation of sago starch using gel permeation chromatography multi-angle laser light scattering |
| title_sort | molecular characterisation of sago starch using gel permeation chromatography multi-angle laser light scattering |
| url | http://journalarticle.ukm.my/7441/1/26_Ayiesah.pdf http://journalarticle.ukm.my/7441/ http://www.ukm.my/jsm/english_journals/vol39num6_2010/contentsVol39num6_2010.html |
| url_provider | http://journalarticle.ukm.my/ |