Epitope mapping of Anti-YB-1 antibodies using truncated proteins
Increased expression and nuclear localization of Y-box-binding protein-1 (YB-1) are associated with drug resistance and tumor progression. Detection of YB-1 primarily depends on the recognition of YB-1 antibody on its epitopes. To determine the epitopes binding of anti-YB-1 A-16 and 59-Q antibodies,...
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| Format: | Article |
| Language: | en |
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Penerbit Universiti Kebangsaan Malaysia
2014
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| Online Access: | http://journalarticle.ukm.my/7285/1/43_1_03.pdf http://journalarticle.ukm.my/7285/ http://mabjournal.com/ |
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| author | Wei, T.C. Shamsuddin, S. |
| author_facet | Wei, T.C. Shamsuddin, S. |
| author_sort | Wei, T.C. |
| building | Tun Sri Lanang Library |
| collection | Institutional Repository |
| content_provider | Universiti Kebangsaan Malaysia |
| content_source | UKM Journal Article Repository |
| continent | Asia |
| country | Malaysia |
| description | Increased expression and nuclear localization of Y-box-binding protein-1 (YB-1) are associated with drug resistance and tumor progression. Detection of YB-1 primarily depends on the recognition of YB-1 antibody on its epitopes. To determine the epitopes binding of anti-YB-1 A-16 and 59-Q antibodies, three YB-1 truncated proteins were generated using bacterial expression system and their aberrant migration were reported. Epitope mapping analyses revealed anti-YB-1 A-16 antibody recognized epitope at 1-127 amino acid residues of YB-1, whereas 59-Q antibody was reactive with epitope at 61-127 amino acid residues. Based on our findings, 59-Q antibody was suggested used in the future study to avoid ambiguity. |
| format | Article |
| id | my-ukm.journal-7285 |
| institution | Universiti Kebangsaan Malaysia |
| language | en |
| publishDate | 2014 |
| publisher | Penerbit Universiti Kebangsaan Malaysia |
| record_format | eprints |
| spelling | my-ukm.journal-72852016-12-14T06:43:38Z http://journalarticle.ukm.my/7285/ Epitope mapping of Anti-YB-1 antibodies using truncated proteins Wei, T.C. Shamsuddin, S. Increased expression and nuclear localization of Y-box-binding protein-1 (YB-1) are associated with drug resistance and tumor progression. Detection of YB-1 primarily depends on the recognition of YB-1 antibody on its epitopes. To determine the epitopes binding of anti-YB-1 A-16 and 59-Q antibodies, three YB-1 truncated proteins were generated using bacterial expression system and their aberrant migration were reported. Epitope mapping analyses revealed anti-YB-1 A-16 antibody recognized epitope at 1-127 amino acid residues of YB-1, whereas 59-Q antibody was reactive with epitope at 61-127 amino acid residues. Based on our findings, 59-Q antibody was suggested used in the future study to avoid ambiguity. Penerbit Universiti Kebangsaan Malaysia 2014-06 Article PeerReviewed application/pdf en http://journalarticle.ukm.my/7285/1/43_1_03.pdf Wei, T.C. and Shamsuddin, S. (2014) Epitope mapping of Anti-YB-1 antibodies using truncated proteins. Malaysian Applied Biology, 43 (1). pp. 21-30. ISSN 0126-8643 http://mabjournal.com/ |
| spellingShingle | Wei, T.C. Shamsuddin, S. Epitope mapping of Anti-YB-1 antibodies using truncated proteins |
| title | Epitope mapping of Anti-YB-1 antibodies using truncated proteins |
| title_full | Epitope mapping of Anti-YB-1 antibodies using truncated proteins |
| title_fullStr | Epitope mapping of Anti-YB-1 antibodies using truncated proteins |
| title_full_unstemmed | Epitope mapping of Anti-YB-1 antibodies using truncated proteins |
| title_short | Epitope mapping of Anti-YB-1 antibodies using truncated proteins |
| title_sort | epitope mapping of anti-yb-1 antibodies using truncated proteins |
| url | http://journalarticle.ukm.my/7285/1/43_1_03.pdf http://journalarticle.ukm.my/7285/ http://mabjournal.com/ |
| url_provider | http://journalarticle.ukm.my/ |