Optimization of extraction and physicochemical properties of gelatin from pangasius catfish (Pangasius sutchi) skin
In order to optimize the extraction of gelatin from pangasius catfish skin, a response surface method (RSM) involving a Central Composite Design (CCD) was applied. Four variables, namely NaOH concentration (0-0.3 N), acetic acid concentration (0.025-0.125 N), extraction time (2-4 h) and extraction t...
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| Language: | en |
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Universiti Kebangsaan Malaysia
2014
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| Online Access: | http://journalarticle.ukm.my/7254/1/05_Fatemeh.pdf http://journalarticle.ukm.my/7254/ http://www.ukm.my/jsm/ |
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| author | Mahmoodani, Fatemeh Ardekani, Venus Sanaei See, Siau Fern Salma Mohamad Yusop, Abdul Salam Babji, |
| author_facet | Mahmoodani, Fatemeh Ardekani, Venus Sanaei See, Siau Fern Salma Mohamad Yusop, Abdul Salam Babji, |
| author_sort | Mahmoodani, Fatemeh |
| building | Tun Sri Lanang Library |
| collection | Institutional Repository |
| content_provider | Universiti Kebangsaan Malaysia |
| content_source | UKM Journal Article Repository |
| continent | Asia |
| country | Malaysia |
| description | In order to optimize the extraction of gelatin from pangasius catfish skin, a response surface method (RSM) involving a Central Composite Design (CCD) was applied. Four variables, namely NaOH concentration (0-0.3 N), acetic acid concentration (0.025-0.125 N), extraction time (2-4 h) and extraction temperature (40-80°C) were selected as independent variables for the optimization using RSM. The dependent variable was calculated by hydroxyproline recovery. The optimum conditions for extraction were produced by a pre-treatment of 0.2 N NaOH and 0.1 N acetic acid along with hot water extraction at 63.7°C for 2.41 h. The results showed that the predicted response by RSM (68.53%) closely matched the experimental response of 68.16%. The results indicated that the extracted gelatin possessed high gel strength (438 g) and high content of imino acid (proline and hydroxyproline) (18.01%) with a viscosity of 4.67 mPa s. The results showed that RSM was a great optimizing tool for extraction of gelatin from pangasius catfish skin. The gelatin was also proven to have significantly (p<0.05) higher quality of physicochemical properties than those from bovine skin gelatin. |
| format | Article |
| id | my-ukm.journal-7254 |
| institution | Universiti Kebangsaan Malaysia |
| language | en |
| publishDate | 2014 |
| publisher | Universiti Kebangsaan Malaysia |
| record_format | eprints |
| spelling | my-ukm.journal-72542016-12-14T06:43:32Z http://journalarticle.ukm.my/7254/ Optimization of extraction and physicochemical properties of gelatin from pangasius catfish (Pangasius sutchi) skin Mahmoodani, Fatemeh Ardekani, Venus Sanaei See, Siau Fern Salma Mohamad Yusop, Abdul Salam Babji, In order to optimize the extraction of gelatin from pangasius catfish skin, a response surface method (RSM) involving a Central Composite Design (CCD) was applied. Four variables, namely NaOH concentration (0-0.3 N), acetic acid concentration (0.025-0.125 N), extraction time (2-4 h) and extraction temperature (40-80°C) were selected as independent variables for the optimization using RSM. The dependent variable was calculated by hydroxyproline recovery. The optimum conditions for extraction were produced by a pre-treatment of 0.2 N NaOH and 0.1 N acetic acid along with hot water extraction at 63.7°C for 2.41 h. The results showed that the predicted response by RSM (68.53%) closely matched the experimental response of 68.16%. The results indicated that the extracted gelatin possessed high gel strength (438 g) and high content of imino acid (proline and hydroxyproline) (18.01%) with a viscosity of 4.67 mPa s. The results showed that RSM was a great optimizing tool for extraction of gelatin from pangasius catfish skin. The gelatin was also proven to have significantly (p<0.05) higher quality of physicochemical properties than those from bovine skin gelatin. Universiti Kebangsaan Malaysia 2014-07 Article PeerReviewed application/pdf en http://journalarticle.ukm.my/7254/1/05_Fatemeh.pdf Mahmoodani, Fatemeh and Ardekani, Venus Sanaei and See, Siau Fern and Salma Mohamad Yusop, and Abdul Salam Babji, (2014) Optimization of extraction and physicochemical properties of gelatin from pangasius catfish (Pangasius sutchi) skin. Sains Malaysiana, 43 (7). pp. 995-1002. ISSN 0126-6039 http://www.ukm.my/jsm/ |
| spellingShingle | Mahmoodani, Fatemeh Ardekani, Venus Sanaei See, Siau Fern Salma Mohamad Yusop, Abdul Salam Babji, Optimization of extraction and physicochemical properties of gelatin from pangasius catfish (Pangasius sutchi) skin |
| title | Optimization of extraction and physicochemical properties of gelatin from pangasius catfish (Pangasius sutchi) skin |
| title_full | Optimization of extraction and physicochemical properties of gelatin from pangasius catfish (Pangasius sutchi) skin |
| title_fullStr | Optimization of extraction and physicochemical properties of gelatin from pangasius catfish (Pangasius sutchi) skin |
| title_full_unstemmed | Optimization of extraction and physicochemical properties of gelatin from pangasius catfish (Pangasius sutchi) skin |
| title_short | Optimization of extraction and physicochemical properties of gelatin from pangasius catfish (Pangasius sutchi) skin |
| title_sort | optimization of extraction and physicochemical properties of gelatin from pangasius catfish (pangasius sutchi) skin |
| url | http://journalarticle.ukm.my/7254/1/05_Fatemeh.pdf http://journalarticle.ukm.my/7254/ http://www.ukm.my/jsm/ |
| url_provider | http://journalarticle.ukm.my/ |