In vitro regeneration and acclimatization protocols of selected ornamental plants (agapanthus praecox, justicia betonica and celosia cristata)

This paper discussed on the effectiveness of BAP and NAA growth hormones on establishment of plant regeneration for selected ornamentals; Agapanthus praecox, Justicia betonica and Celosia cristata. Various explants (leaf, stem, shoot tip and bulb) derived from one-month-old aseptic seedlings of A. p...

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Main Authors: Jamilah Syafawati Yaacob, Azani Saleh, Hashimah Elias, Sakinah Abdullah, Noraini Mahmad, Normadiha Mohamed
Format: Article
Language:en
Published: Universiti Kebangsaan Malaysia 2014
Online Access:http://journalarticle.ukm.my/7152/1/09_Jamilah_Syafawati.pdf
http://journalarticle.ukm.my/7152/
http://www.ukm.my/jsm/
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author Jamilah Syafawati Yaacob,
Azani Saleh,
Hashimah Elias,
Sakinah Abdullah,
Noraini Mahmad,
Normadiha Mohamed,
author_facet Jamilah Syafawati Yaacob,
Azani Saleh,
Hashimah Elias,
Sakinah Abdullah,
Noraini Mahmad,
Normadiha Mohamed,
author_sort Jamilah Syafawati Yaacob,
building Tun Sri Lanang Library
collection Institutional Repository
content_provider Universiti Kebangsaan Malaysia
content_source UKM Journal Article Repository
continent Asia
country Malaysia
description This paper discussed on the effectiveness of BAP and NAA growth hormones on establishment of plant regeneration for selected ornamentals; Agapanthus praecox, Justicia betonica and Celosia cristata. Various explants (leaf, stem, shoot tip and bulb) derived from one-month-old aseptic seedlings of A. praecox and C. cristata, as well as explants from intact plants of J. betonica were utilized to achieve complete plant regeneration of these species. MS medium supplemented with various hormones, with an emphasis on BAP and NAA was tested to obtain direct and indirect regeneration. Both A. praecox (bulbs) and C. cristata (shoots) formed complete plantlets on MS added with 0.5-2.0 mg/L BAP and NAA, while direct regeneration was achieved for J. betonica on MS media containing BAP. Several methods were attempted to acclimatize the regenerants, with A. praecox gave the highest percentage of survival rates (96.67%), followed by J. betonica (80.00%) and C. cristata (75.00%).
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institution Universiti Kebangsaan Malaysia
language en
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publisher Universiti Kebangsaan Malaysia
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spelling my-ukm.journal-71522016-12-14T06:43:14Z http://journalarticle.ukm.my/7152/ In vitro regeneration and acclimatization protocols of selected ornamental plants (agapanthus praecox, justicia betonica and celosia cristata) Jamilah Syafawati Yaacob, Azani Saleh, Hashimah Elias, Sakinah Abdullah, Noraini Mahmad, Normadiha Mohamed, This paper discussed on the effectiveness of BAP and NAA growth hormones on establishment of plant regeneration for selected ornamentals; Agapanthus praecox, Justicia betonica and Celosia cristata. Various explants (leaf, stem, shoot tip and bulb) derived from one-month-old aseptic seedlings of A. praecox and C. cristata, as well as explants from intact plants of J. betonica were utilized to achieve complete plant regeneration of these species. MS medium supplemented with various hormones, with an emphasis on BAP and NAA was tested to obtain direct and indirect regeneration. Both A. praecox (bulbs) and C. cristata (shoots) formed complete plantlets on MS added with 0.5-2.0 mg/L BAP and NAA, while direct regeneration was achieved for J. betonica on MS media containing BAP. Several methods were attempted to acclimatize the regenerants, with A. praecox gave the highest percentage of survival rates (96.67%), followed by J. betonica (80.00%) and C. cristata (75.00%). Universiti Kebangsaan Malaysia 2014-05 Article PeerReviewed application/pdf en http://journalarticle.ukm.my/7152/1/09_Jamilah_Syafawati.pdf Jamilah Syafawati Yaacob, and Azani Saleh, and Hashimah Elias, and Sakinah Abdullah, and Noraini Mahmad, and Normadiha Mohamed, (2014) In vitro regeneration and acclimatization protocols of selected ornamental plants (agapanthus praecox, justicia betonica and celosia cristata). Sains Malaysiana, 43 (5). pp. 715-722. ISSN 0126-6039 http://www.ukm.my/jsm/
spellingShingle Jamilah Syafawati Yaacob,
Azani Saleh,
Hashimah Elias,
Sakinah Abdullah,
Noraini Mahmad,
Normadiha Mohamed,
In vitro regeneration and acclimatization protocols of selected ornamental plants (agapanthus praecox, justicia betonica and celosia cristata)
title In vitro regeneration and acclimatization protocols of selected ornamental plants (agapanthus praecox, justicia betonica and celosia cristata)
title_full In vitro regeneration and acclimatization protocols of selected ornamental plants (agapanthus praecox, justicia betonica and celosia cristata)
title_fullStr In vitro regeneration and acclimatization protocols of selected ornamental plants (agapanthus praecox, justicia betonica and celosia cristata)
title_full_unstemmed In vitro regeneration and acclimatization protocols of selected ornamental plants (agapanthus praecox, justicia betonica and celosia cristata)
title_short In vitro regeneration and acclimatization protocols of selected ornamental plants (agapanthus praecox, justicia betonica and celosia cristata)
title_sort in vitro regeneration and acclimatization protocols of selected ornamental plants (agapanthus praecox, justicia betonica and celosia cristata)
url http://journalarticle.ukm.my/7152/1/09_Jamilah_Syafawati.pdf
http://journalarticle.ukm.my/7152/
http://www.ukm.my/jsm/
url_provider http://journalarticle.ukm.my/