In vitro shoot regeneration from leaf explants of kenaf (Hibiscus cannabinus L.)
Kenaf (Hibiscus cannabinus L.) is a versatile plant with multiuse ranging from animal feed to a wide variety of biocomposite products such as pulp and paper and fibre reinforce plastic. Therefore genetically improved planting materials are needed to tailor made requirement of the industry. Thus, dev...
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| Format: | Article |
| Language: | en |
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Universiti Kebangsaan Malaysia
2013
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| Online Access: | http://journalarticle.ukm.my/6554/1/21_P.W._Samanthi.pdf http://journalarticle.ukm.my/6554/ http://www.ukm.my/jsm/ |
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| author | Samanthi, P.W. Mohd Puad, A. Suhaimi, N. Kumar, S.M. Nor Aini, A.S. |
| author_facet | Samanthi, P.W. Mohd Puad, A. Suhaimi, N. Kumar, S.M. Nor Aini, A.S. |
| author_sort | Samanthi, P.W. |
| building | Tun Sri Lanang Library |
| collection | Institutional Repository |
| content_provider | Universiti Kebangsaan Malaysia |
| content_source | UKM Journal Article Repository |
| continent | Asia |
| country | Malaysia |
| description | Kenaf (Hibiscus cannabinus L.) is a versatile plant with multiuse ranging from animal feed to a wide variety of biocomposite products such as pulp and paper and fibre reinforce plastic. Therefore genetically improved planting materials are needed to tailor made requirement of the industry. Thus, development of plant regeneration through callus is important for in vitro genetic manipulation of kenaf. Currently development of successful genetic transformation of kenaf is through in planta transformation means. In vitro shoot regeneration was conducted using leaf explants from varieties V36 and G4 treated to three different combinations of N6 Benzyl adenine (BA) and Indole-3-butyric acid (IBA). High percentage of healthy callus induction was produced in MS medium supplemented with combination of 1.5 mgL-1 BA and 0.5 mgL-1 IBA. In addition 68.7% plant regeneration was obtained in MS medium supplemented with 0.3 mgL-1 GA3. All plantlets produced roots in hormone free medium. There was no significant difference among varieties in terms of callus induction (number of callus) and plant regeneration (number of plantlets). This protocol is useful to be used for the development of gene transformation protocol of kenaf through callus. |
| format | Article |
| id | my-ukm.journal-6554 |
| institution | Universiti Kebangsaan Malaysia |
| language | en |
| publishDate | 2013 |
| publisher | Universiti Kebangsaan Malaysia |
| record_format | eprints |
| spelling | my-ukm.journal-65542016-12-14T06:41:31Z http://journalarticle.ukm.my/6554/ In vitro shoot regeneration from leaf explants of kenaf (Hibiscus cannabinus L.) Samanthi, P.W. Mohd Puad, A. Suhaimi, N. Kumar, S.M. Nor Aini, A.S. Kenaf (Hibiscus cannabinus L.) is a versatile plant with multiuse ranging from animal feed to a wide variety of biocomposite products such as pulp and paper and fibre reinforce plastic. Therefore genetically improved planting materials are needed to tailor made requirement of the industry. Thus, development of plant regeneration through callus is important for in vitro genetic manipulation of kenaf. Currently development of successful genetic transformation of kenaf is through in planta transformation means. In vitro shoot regeneration was conducted using leaf explants from varieties V36 and G4 treated to three different combinations of N6 Benzyl adenine (BA) and Indole-3-butyric acid (IBA). High percentage of healthy callus induction was produced in MS medium supplemented with combination of 1.5 mgL-1 BA and 0.5 mgL-1 IBA. In addition 68.7% plant regeneration was obtained in MS medium supplemented with 0.3 mgL-1 GA3. All plantlets produced roots in hormone free medium. There was no significant difference among varieties in terms of callus induction (number of callus) and plant regeneration (number of plantlets). This protocol is useful to be used for the development of gene transformation protocol of kenaf through callus. Universiti Kebangsaan Malaysia 2013-10 Article PeerReviewed application/pdf en http://journalarticle.ukm.my/6554/1/21_P.W._Samanthi.pdf Samanthi, P.W. and Mohd Puad, A. and Suhaimi, N. and Kumar, S.M. and Nor Aini, A.S. (2013) In vitro shoot regeneration from leaf explants of kenaf (Hibiscus cannabinus L.). Sains Malaysiana, 42 (10). pp. 1505-1510. ISSN 0126-6039 http://www.ukm.my/jsm/ |
| spellingShingle | Samanthi, P.W. Mohd Puad, A. Suhaimi, N. Kumar, S.M. Nor Aini, A.S. In vitro shoot regeneration from leaf explants of kenaf (Hibiscus cannabinus L.) |
| title | In vitro shoot regeneration from leaf explants of kenaf
(Hibiscus cannabinus L.) |
| title_full | In vitro shoot regeneration from leaf explants of kenaf
(Hibiscus cannabinus L.) |
| title_fullStr | In vitro shoot regeneration from leaf explants of kenaf
(Hibiscus cannabinus L.) |
| title_full_unstemmed | In vitro shoot regeneration from leaf explants of kenaf
(Hibiscus cannabinus L.) |
| title_short | In vitro shoot regeneration from leaf explants of kenaf
(Hibiscus cannabinus L.) |
| title_sort | in vitro shoot regeneration from leaf explants of kenaf
(hibiscus cannabinus l.) |
| url | http://journalarticle.ukm.my/6554/1/21_P.W._Samanthi.pdf http://journalarticle.ukm.my/6554/ http://www.ukm.my/jsm/ |
| url_provider | http://journalarticle.ukm.my/ |