Optimal physical and nutrient parameters for growth of trichoderma virens UKMP-1M for heavy crude oil degradation (Pengoptimuman Parameter Fizikal dan nutrien bagi pertumbuhan trichoderma virens UKMP-1M untuk degradasi minyak mentah berat)
This study was carried out to determine the optimal parameters for the production of biomass of Trichoderma virens UKMP-1M, a fungus isolated from oil-polluted wastewater. The isolate showed maximum growth at day six after incubation in Mineral Salt Medium (MSM) in the presence of 3% (v/v) heavy Khe...
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Universiti Kebangsaan Malaysia
2012
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| Online Access: | http://journalarticle.ukm.my/3232/1/09_Ainon_Hamzah.pdf http://journalarticle.ukm.my/3232/ http://www.ukm.my/jsm/contents.html |
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| author | Ainon Hamzah, Mazni Abu Zain, Othman Omar, Sahidan Senafi, |
| author_facet | Ainon Hamzah, Mazni Abu Zain, Othman Omar, Sahidan Senafi, |
| author_sort | Ainon Hamzah, |
| building | Tun Sri Lanang Library |
| collection | Institutional Repository |
| content_provider | Universiti Kebangsaan Malaysia |
| content_source | UKM Journal Article Repository |
| continent | Asia |
| country | Malaysia |
| description | This study was carried out to determine the optimal parameters for the production of biomass of Trichoderma virens UKMP-1M, a fungus isolated from oil-polluted wastewater. The isolate showed maximum growth at day six after incubation in Mineral Salt Medium (MSM) in the presence of 3% (v/v) heavy Khefji Sour crude oil. Although it grew at pH between 5.0 and 7.0, it grew best at pH 5.5. T. virens UKMP-1M grew at temperatures between 25°C and 35°C, with its highest growth at 30°C. Aeration by agitation at 200 rpm was shown to yield the greatest biomass. Peptone at concentration of 1.5% (w/v) was determined to be a better nitrogen source than urea, potassium nitrate (KNO3), yeast extract, ammonium sulphate ((NH4)2SO4) and ammonium chloride (NH4Cl). Addition of 1% (v/v) crude oil to the MSM medium led to higher biomass production than the addition of 3%, 5%, 7% and 10% (v/v) crude oil. The result also revealed that 40% of total petroleum hydrocarbon (TPH), 100% of pristane and 74% of phytane compounds were degraded after 9 days of incubation at optimal physical and nutrient parameters. |
| format | Article |
| id | my-ukm.journal-3232 |
| institution | Universiti Kebangsaan Malaysia |
| language | en |
| publishDate | 2012 |
| publisher | Universiti Kebangsaan Malaysia |
| record_format | eprints |
| spelling | my-ukm.journal-32322016-12-14T06:34:01Z http://journalarticle.ukm.my/3232/ Optimal physical and nutrient parameters for growth of trichoderma virens UKMP-1M for heavy crude oil degradation (Pengoptimuman Parameter Fizikal dan nutrien bagi pertumbuhan trichoderma virens UKMP-1M untuk degradasi minyak mentah berat) Ainon Hamzah, Mazni Abu Zain, Othman Omar, Sahidan Senafi, This study was carried out to determine the optimal parameters for the production of biomass of Trichoderma virens UKMP-1M, a fungus isolated from oil-polluted wastewater. The isolate showed maximum growth at day six after incubation in Mineral Salt Medium (MSM) in the presence of 3% (v/v) heavy Khefji Sour crude oil. Although it grew at pH between 5.0 and 7.0, it grew best at pH 5.5. T. virens UKMP-1M grew at temperatures between 25°C and 35°C, with its highest growth at 30°C. Aeration by agitation at 200 rpm was shown to yield the greatest biomass. Peptone at concentration of 1.5% (w/v) was determined to be a better nitrogen source than urea, potassium nitrate (KNO3), yeast extract, ammonium sulphate ((NH4)2SO4) and ammonium chloride (NH4Cl). Addition of 1% (v/v) crude oil to the MSM medium led to higher biomass production than the addition of 3%, 5%, 7% and 10% (v/v) crude oil. The result also revealed that 40% of total petroleum hydrocarbon (TPH), 100% of pristane and 74% of phytane compounds were degraded after 9 days of incubation at optimal physical and nutrient parameters. Universiti Kebangsaan Malaysia 2012-01 Article PeerReviewed application/pdf en http://journalarticle.ukm.my/3232/1/09_Ainon_Hamzah.pdf Ainon Hamzah, and Mazni Abu Zain, and Othman Omar, and Sahidan Senafi, (2012) Optimal physical and nutrient parameters for growth of trichoderma virens UKMP-1M for heavy crude oil degradation (Pengoptimuman Parameter Fizikal dan nutrien bagi pertumbuhan trichoderma virens UKMP-1M untuk degradasi minyak mentah berat). Sains Malaysiana, 41 (1). pp. 71-79. ISSN 0126-6039 http://www.ukm.my/jsm/contents.html |
| spellingShingle | Ainon Hamzah, Mazni Abu Zain, Othman Omar, Sahidan Senafi, Optimal physical and nutrient parameters for growth of trichoderma virens UKMP-1M for heavy crude oil degradation (Pengoptimuman Parameter Fizikal dan nutrien bagi pertumbuhan trichoderma virens UKMP-1M untuk degradasi minyak mentah berat) |
| title | Optimal physical and nutrient parameters for growth of trichoderma virens UKMP-1M for heavy crude oil degradation
(Pengoptimuman Parameter Fizikal dan nutrien bagi pertumbuhan trichoderma virens UKMP-1M untuk degradasi minyak mentah berat) |
| title_full | Optimal physical and nutrient parameters for growth of trichoderma virens UKMP-1M for heavy crude oil degradation
(Pengoptimuman Parameter Fizikal dan nutrien bagi pertumbuhan trichoderma virens UKMP-1M untuk degradasi minyak mentah berat) |
| title_fullStr | Optimal physical and nutrient parameters for growth of trichoderma virens UKMP-1M for heavy crude oil degradation
(Pengoptimuman Parameter Fizikal dan nutrien bagi pertumbuhan trichoderma virens UKMP-1M untuk degradasi minyak mentah berat) |
| title_full_unstemmed | Optimal physical and nutrient parameters for growth of trichoderma virens UKMP-1M for heavy crude oil degradation
(Pengoptimuman Parameter Fizikal dan nutrien bagi pertumbuhan trichoderma virens UKMP-1M untuk degradasi minyak mentah berat) |
| title_short | Optimal physical and nutrient parameters for growth of trichoderma virens UKMP-1M for heavy crude oil degradation
(Pengoptimuman Parameter Fizikal dan nutrien bagi pertumbuhan trichoderma virens UKMP-1M untuk degradasi minyak mentah berat) |
| title_sort | optimal physical and nutrient parameters for growth of trichoderma virens ukmp-1m for heavy crude oil degradation
(pengoptimuman parameter fizikal dan nutrien bagi pertumbuhan trichoderma virens ukmp-1m untuk degradasi minyak mentah berat) |
| url | http://journalarticle.ukm.my/3232/1/09_Ainon_Hamzah.pdf http://journalarticle.ukm.my/3232/ http://www.ukm.my/jsm/contents.html |
| url_provider | http://journalarticle.ukm.my/ |