Computational studies on the cognate and non-cognate aminoacyl-tRNAs
Although kinetic proofreading plays a major role in the fidelity of protein synthesis, it only includes the codon-anticodon interaction without considering the amino acid side chain. It is not known whether the ribosome has a specific interaction with the amino acid side chain or if the correctly ch...
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Faculty of Science, University of Malaya
2011
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my.um.eprints.60682021-04-27T08:09:27Z http://eprints.um.edu.my/6068/ Computational studies on the cognate and non-cognate aminoacyl-tRNAs Monajemi, H. Abdullah, W.A.T.W. Zain, Sharifuddin Md QD Chemistry Although kinetic proofreading plays a major role in the fidelity of protein synthesis, it only includes the codon-anticodon interaction without considering the amino acid side chain. It is not known whether the ribosome has a specific interaction with the amino acid side chain or if the correctly charged tRNA behaves in such a way that brings this fidelity to protein synthesis process. To answer this question, we performed some preliminary calculations using numerical analysis on the misacylated tRNAs and compared the results with that of the cognate aminoacyl-tRNAs. Due to the large size of the molecule, we used 2-layered ONIOM (QM/MM) calculations. Interestingly, it was observed that only the initiator tRNA, mat has a specific interaction with the amino acid side chain. Faculty of Science, University of Malaya 2011 Article PeerReviewed Monajemi, H. and Abdullah, W.A.T.W. and Zain, Sharifuddin Md (2011) Computational studies on the cognate and non-cognate aminoacyl-tRNAs. Malaysian Journal of Science, 30 (2). pp. 127-132. ISSN 1394-3065 http://www.scopus.com/inward/record.url?eid=2-s2.0-80054122918&partnerID=40&md5=cf93825b877dcc9e9576c81900f3d45d |
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QD Chemistry Monajemi, H. Abdullah, W.A.T.W. Zain, Sharifuddin Md Computational studies on the cognate and non-cognate aminoacyl-tRNAs |
| description |
Although kinetic proofreading plays a major role in the fidelity of protein synthesis, it only includes the codon-anticodon interaction without considering the amino acid side chain. It is not known whether the ribosome has a specific interaction with the amino acid side chain or if the correctly charged tRNA behaves in such a way that brings this fidelity to protein synthesis process. To answer this question, we performed some preliminary calculations using numerical analysis on the misacylated tRNAs and compared the results with that of the cognate aminoacyl-tRNAs. Due to the large size of the molecule, we used 2-layered ONIOM (QM/MM) calculations. Interestingly, it was observed that only the initiator tRNA, mat has a specific interaction with the amino acid side chain. |
| format |
Article |
| author |
Monajemi, H. Abdullah, W.A.T.W. Zain, Sharifuddin Md |
| author_facet |
Monajemi, H. Abdullah, W.A.T.W. Zain, Sharifuddin Md |
| author_sort |
Monajemi, H. |
| title |
Computational studies on the cognate and non-cognate aminoacyl-tRNAs |
| title_short |
Computational studies on the cognate and non-cognate aminoacyl-tRNAs |
| title_full |
Computational studies on the cognate and non-cognate aminoacyl-tRNAs |
| title_fullStr |
Computational studies on the cognate and non-cognate aminoacyl-tRNAs |
| title_full_unstemmed |
Computational studies on the cognate and non-cognate aminoacyl-tRNAs |
| title_sort |
computational studies on the cognate and non-cognate aminoacyl-trnas |
| publisher |
Faculty of Science, University of Malaya |
| publishDate |
2011 |
| url |
http://eprints.um.edu.my/6068/ http://www.scopus.com/inward/record.url?eid=2-s2.0-80054122918&partnerID=40&md5=cf93825b877dcc9e9576c81900f3d45d |
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13.250246 |