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Study on the mobile phase composition and flow rate parameter for tributyrin using high performance liquid chromatography-uv detector (HPLC-UV) / Fariswan Atan

The chromatographic separation for standard tributyrin was done by using high performance liquid chromatography (HPLC) equipped with ultraviolet (UV) detector. 20 µL of tributyrin standard with concentration of 0.018 rnM was injected into HPLC attached to the column Carbonl8 (Cl8) at 215 nm waveleng...

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Main Author: Atan, Fariswan
Format: Student Project
Language:English
Published: 2019
Subjects:
Laboratories. General works
Physical and theoretical chemistry
TP Chemical technology
Online Access:http://ir.uitm.edu.my/id/eprint/42449/1/42449.pdf
http://ir.uitm.edu.my/id/eprint/42449/
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spelling my.uitm.ir.424492021-02-26T02:46:13Z http://ir.uitm.edu.my/id/eprint/42449/ Study on the mobile phase composition and flow rate parameter for tributyrin using high performance liquid chromatography-uv detector (HPLC-UV) / Fariswan Atan Atan, Fariswan Laboratories. General works Physical and theoretical chemistry TP Chemical technology The chromatographic separation for standard tributyrin was done by using high performance liquid chromatography (HPLC) equipped with ultraviolet (UV) detector. 20 µL of tributyrin standard with concentration of 0.018 rnM was injected into HPLC attached to the column Carbonl8 (Cl8) at 215 nm wavelength. Two optimization of HPLC parameters have been done in order to study the best condition for tributyrin elution. Two parameters were studied for the elution of tributyrin which are mobile phase composition and flowrate. It was found that the optimum mobile phase composition for tributyin was 50:50 acetonitrile:acetone and the optimum flowrate for tributyrin was at 0.55 mL/min. The calibration curve of standard tributyrin's range was found proportionally linear to tributyrin concentration from 0.009-0.075 mM and the value of R2 was 0.9502. Limit of detection (LOD) and limit of quantitation (LOQ) also have been conducted in this study. The value of LOD and LOQ obtained from the calculation were 0.0032 rnM and 0.0096 mM respectively. 2019 Student Project NonPeerReviewed text en http://ir.uitm.edu.my/id/eprint/42449/1/42449.pdf Atan, Fariswan (2019) Study on the mobile phase composition and flow rate parameter for tributyrin using high performance liquid chromatography-uv detector (HPLC-UV) / Fariswan Atan. [Student Project] (Unpublished)
institution Universiti Teknologi Mara
building Tun Abdul Razak Library
collection Institutional Repository
continent Asia
country Malaysia
content_provider Universiti Teknologi Mara
content_source UiTM Institutional Repository
url_provider http://ir.uitm.edu.my/
language English
topic Laboratories. General works
Physical and theoretical chemistry
TP Chemical technology
spellingShingle Laboratories. General works
Physical and theoretical chemistry
TP Chemical technology
Atan, Fariswan
Study on the mobile phase composition and flow rate parameter for tributyrin using high performance liquid chromatography-uv detector (HPLC-UV) / Fariswan Atan
description The chromatographic separation for standard tributyrin was done by using high performance liquid chromatography (HPLC) equipped with ultraviolet (UV) detector. 20 µL of tributyrin standard with concentration of 0.018 rnM was injected into HPLC attached to the column Carbonl8 (Cl8) at 215 nm wavelength. Two optimization of HPLC parameters have been done in order to study the best condition for tributyrin elution. Two parameters were studied for the elution of tributyrin which are mobile phase composition and flowrate. It was found that the optimum mobile phase composition for tributyin was 50:50 acetonitrile:acetone and the optimum flowrate for tributyrin was at 0.55 mL/min. The calibration curve of standard tributyrin's range was found proportionally linear to tributyrin concentration from 0.009-0.075 mM and the value of R2 was 0.9502. Limit of detection (LOD) and limit of quantitation (LOQ) also have been conducted in this study. The value of LOD and LOQ obtained from the calculation were 0.0032 rnM and 0.0096 mM respectively.
format Student Project
author Atan, Fariswan
author_facet Atan, Fariswan
author_sort Atan, Fariswan
title Study on the mobile phase composition and flow rate parameter for tributyrin using high performance liquid chromatography-uv detector (HPLC-UV) / Fariswan Atan
title_short Study on the mobile phase composition and flow rate parameter for tributyrin using high performance liquid chromatography-uv detector (HPLC-UV) / Fariswan Atan
title_full Study on the mobile phase composition and flow rate parameter for tributyrin using high performance liquid chromatography-uv detector (HPLC-UV) / Fariswan Atan
title_fullStr Study on the mobile phase composition and flow rate parameter for tributyrin using high performance liquid chromatography-uv detector (HPLC-UV) / Fariswan Atan
title_full_unstemmed Study on the mobile phase composition and flow rate parameter for tributyrin using high performance liquid chromatography-uv detector (HPLC-UV) / Fariswan Atan
title_sort study on the mobile phase composition and flow rate parameter for tributyrin using high performance liquid chromatography-uv detector (hplc-uv) / fariswan atan
publishDate 2019
url http://ir.uitm.edu.my/id/eprint/42449/1/42449.pdf
http://ir.uitm.edu.my/id/eprint/42449/
_version_ 1692994698560405504
score 13.251813

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