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The chromatographic study of the acetone extract of fagopyrum / Nur Amirah Zulkepli

Two samples of Fagopyrum species which are Fagopyrum esculentum Moench (common buckwheat) and Fagopyrum tataricum L. Gaertn (tartary buckwheat) were studied for their botanical and phytochemical aspects. In this research, the botanical aspect of Fagopyrum was introduced while the phytochemical aspec...

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Main Author: Zulkepli, Nur Amirah
Format: Thesis
Language:English
Published: 2013
Subjects:
Pharmacopoeias
Online Access:https://ir.uitm.edu.my/id/eprint/109650/1/109650.PDF
https://ir.uitm.edu.my/id/eprint/109650/
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spelling my.uitm.ir.1096502025-02-14T12:46:59Z https://ir.uitm.edu.my/id/eprint/109650/ The chromatographic study of the acetone extract of fagopyrum / Nur Amirah Zulkepli Zulkepli, Nur Amirah Pharmacopoeias Two samples of Fagopyrum species which are Fagopyrum esculentum Moench (common buckwheat) and Fagopyrum tataricum L. Gaertn (tartary buckwheat) were studied for their botanical and phytochemical aspects. In this research, the botanical aspect of Fagopyrum was introduced while the phytochemical aspect was investigated by thin layer chromatography (TLC), in order to detect the chemical constituents present in the seed extracts. The methodology included the isolation, purification and identification of its phytochemical constituents. The purification of the acetone extract was done by using analytical TLC and preparative TLC. The compounds of interest were subjected to nuclear magnetic resonance (NMR) to identify their structure. The phytochemical that was successfully purified was identified as a fatty acid derivative. In addition, this result was supported by the literature that mentioned the isolation of unsaturated fatty acids, such as oleic (18: l ), linoleic (18 :2) and linolenic (18: 3) acids. Linoleic acid was reported as the major polyunsaturated fatty acid of the buckwheat lipid. In conclusion, the Proton NMR (1H-NMR) spectroscopy also provided useful data for analyzing fatty acid composition since the quantitation of each fatty acid signal was carried out by evaluating the particular peaks. 2013 Thesis NonPeerReviewed text en https://ir.uitm.edu.my/id/eprint/109650/1/109650.PDF The chromatographic study of the acetone extract of fagopyrum / Nur Amirah Zulkepli. (2013) Degree thesis, thesis, Universiti Teknologi MARA (Kampus Puncak Alam).
institution Universiti Teknologi Mara
building Tun Abdul Razak Library
collection Institutional Repository
continent Asia
country Malaysia
content_provider Universiti Teknologi Mara
content_source UiTM Institutional Repository
url_provider http://ir.uitm.edu.my/
language English
topic Pharmacopoeias
spellingShingle Pharmacopoeias
Zulkepli, Nur Amirah
The chromatographic study of the acetone extract of fagopyrum / Nur Amirah Zulkepli
description Two samples of Fagopyrum species which are Fagopyrum esculentum Moench (common buckwheat) and Fagopyrum tataricum L. Gaertn (tartary buckwheat) were studied for their botanical and phytochemical aspects. In this research, the botanical aspect of Fagopyrum was introduced while the phytochemical aspect was investigated by thin layer chromatography (TLC), in order to detect the chemical constituents present in the seed extracts. The methodology included the isolation, purification and identification of its phytochemical constituents. The purification of the acetone extract was done by using analytical TLC and preparative TLC. The compounds of interest were subjected to nuclear magnetic resonance (NMR) to identify their structure. The phytochemical that was successfully purified was identified as a fatty acid derivative. In addition, this result was supported by the literature that mentioned the isolation of unsaturated fatty acids, such as oleic (18: l ), linoleic (18 :2) and linolenic (18: 3) acids. Linoleic acid was reported as the major polyunsaturated fatty acid of the buckwheat lipid. In conclusion, the Proton NMR (1H-NMR) spectroscopy also provided useful data for analyzing fatty acid composition since the quantitation of each fatty acid signal was carried out by evaluating the particular peaks.
format Thesis
author Zulkepli, Nur Amirah
author_facet Zulkepli, Nur Amirah
author_sort Zulkepli, Nur Amirah
title The chromatographic study of the acetone extract of fagopyrum / Nur Amirah Zulkepli
title_short The chromatographic study of the acetone extract of fagopyrum / Nur Amirah Zulkepli
title_full The chromatographic study of the acetone extract of fagopyrum / Nur Amirah Zulkepli
title_fullStr The chromatographic study of the acetone extract of fagopyrum / Nur Amirah Zulkepli
title_full_unstemmed The chromatographic study of the acetone extract of fagopyrum / Nur Amirah Zulkepli
title_sort chromatographic study of the acetone extract of fagopyrum / nur amirah zulkepli
publishDate 2013
url https://ir.uitm.edu.my/id/eprint/109650/1/109650.PDF
https://ir.uitm.edu.my/id/eprint/109650/
_version_ 1825165120364347392
score 13.239859

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